Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8853 | 5' | -55.9 | NC_002484.1 | + | 50750 | 0.66 | 0.690233 |
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Target: 5'- uGCCUCgGUCGCC----GGGaccaGGCCCu -3' miRNA: 3'- -CGGAGgUAGCGGauguUCCa---UCGGGc -5' |
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| 8853 | 5' | -55.9 | NC_002484.1 | + | 7755 | 0.66 | 0.668521 |
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Target: 5'- gGCUUUCGuUCGUCaggGCGGGGUuaggcggcuucuAGCCCGa -3' miRNA: 3'- -CGGAGGU-AGCGGa--UGUUCCA------------UCGGGC- -5' |
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| 8853 | 5' | -55.9 | NC_002484.1 | + | 925 | 0.68 | 0.581125 |
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Target: 5'- aCCUCgAagGCCUGCuucucgguGGUGGCgCCGg -3' miRNA: 3'- cGGAGgUagCGGAUGuu------CCAUCG-GGC- -5' |
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| 8853 | 5' | -55.9 | NC_002484.1 | + | 56228 | 0.68 | 0.563822 |
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Target: 5'- -aCUCCAUUGCCgucuuccucgcaugACAuGGGUAGCCa- -3' miRNA: 3'- cgGAGGUAGCGGa-------------UGU-UCCAUCGGgc -5' |
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| 8853 | 5' | -55.9 | NC_002484.1 | + | 37427 | 1.1 | 0.000728 |
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Target: 5'- cGCCUCCAUCGCCUACAAGGUAGCCCGc -3' miRNA: 3'- -CGGAGGUAGCGGAUGUUCCAUCGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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