Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8854 | 3' | -51.8 | NC_002484.1 | + | 35968 | 0.66 | 0.84604 |
Target: 5'- -cGGcaacgCGGcuGUGCC-GCCAAAGAAGCu -3' miRNA: 3'- aaCCa----GCUu-CACGGcCGGUUUCUUUG- -5' |
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8854 | 3' | -51.8 | NC_002484.1 | + | 34919 | 0.66 | 0.827598 |
Target: 5'- -aGGUUcAAGcGCCGGCCAGAcGAcACc -3' miRNA: 3'- aaCCAGcUUCaCGGCCGGUUU-CUuUG- -5' |
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8854 | 3' | -51.8 | NC_002484.1 | + | 51094 | 0.67 | 0.777786 |
Target: 5'- -aGGUgGAAGUGaCCGGCCuc-GAu-- -3' miRNA: 3'- aaCCAgCUUCAC-GGCCGGuuuCUuug -5' |
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8854 | 3' | -51.8 | NC_002484.1 | + | 14717 | 0.69 | 0.698163 |
Target: 5'- -aGGUCGuAGUaGCCGGCCuccaccuggacaguGAGACu -3' miRNA: 3'- aaCCAGCuUCA-CGGCCGGuuu-----------CUUUG- -5' |
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8854 | 3' | -51.8 | NC_002484.1 | + | 27673 | 0.7 | 0.610211 |
Target: 5'- cUGGcCGucaacacaGGGUGCCGGCgGGAGGAAa -3' miRNA: 3'- aACCaGC--------UUCACGGCCGgUUUCUUUg -5' |
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8854 | 3' | -51.8 | NC_002484.1 | + | 37567 | 1.07 | 0.002593 |
Target: 5'- aUUGGUCGAAGUGCCGGCCAAAGAAACc -3' miRNA: 3'- -AACCAGCUUCACGGCCGGUUUCUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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