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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8855 | 3' | -51.6 | NC_002484.1 | + | 38425 | 1.13 | 0.001432 |
Target: 5'- gCCGAGGUGGAACAUCUACGCAAGGACg -3' miRNA: 3'- -GGCUCCACCUUGUAGAUGCGUUCCUG- -5' |
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8855 | 3' | -51.6 | NC_002484.1 | + | 18250 | 0.7 | 0.713968 |
Target: 5'- gCC-AGGgccgGGAACAUCgccgGCAAGGGCc -3' miRNA: 3'- -GGcUCCa---CCUUGUAGaug-CGUUCCUG- -5' |
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8855 | 3' | -51.6 | NC_002484.1 | + | 39842 | 0.66 | 0.900129 |
Target: 5'- gCCGAGuaacGUGaucAGCAUCgGCaGCAAGGGCg -3' miRNA: 3'- -GGCUC----CACc--UUGUAGaUG-CGUUCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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