Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8856 | 5' | -56.3 | NC_002484.1 | + | 10580 | 0.66 | 0.672463 |
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Target: 5'- cACCACAGCGUUGGCUGCaaaaucgaacgggaaGUcgcucacauagCCGCGg -3' miRNA: 3'- uUGGUGUCGCAGUUGAUG---------------CG-----------GGCGCg -5' |
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| 8856 | 5' | -56.3 | NC_002484.1 | + | 41984 | 0.66 | 0.642848 |
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Target: 5'- uGCCGCGaugacguagcgcguGCGcUCGGCuUGCGCCCgaacgaagaGCGCg -3' miRNA: 3'- uUGGUGU--------------CGC-AGUUG-AUGCGGG---------CGCG- -5' |
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| 8856 | 5' | -56.3 | NC_002484.1 | + | 37003 | 0.66 | 0.613161 |
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Target: 5'- cGACUGC-GCGcCGaaGCUGcCGCCCGCGa -3' miRNA: 3'- -UUGGUGuCGCaGU--UGAU-GCGGGCGCg -5' |
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| 8856 | 5' | -56.3 | NC_002484.1 | + | 13301 | 0.68 | 0.516006 |
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Target: 5'- cAGCCAUugGGCGgcCAuCUGCGCaaGCGCg -3' miRNA: 3'- -UUGGUG--UCGCa-GUuGAUGCGggCGCG- -5' |
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| 8856 | 5' | -56.3 | NC_002484.1 | + | 20010 | 0.7 | 0.388586 |
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Target: 5'- cAGCaCACAGCGUCcAUUcCGCCaaGCGCg -3' miRNA: 3'- -UUG-GUGUCGCAGuUGAuGCGGg-CGCG- -5' |
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| 8856 | 5' | -56.3 | NC_002484.1 | + | 6929 | 0.71 | 0.345359 |
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Target: 5'- -uCCGCAGCGaUCAACUcGCGaCCCG-GCc -3' miRNA: 3'- uuGGUGUCGC-AGUUGA-UGC-GGGCgCG- -5' |
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| 8856 | 5' | -56.3 | NC_002484.1 | + | 28027 | 0.72 | 0.321128 |
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Target: 5'- cGCCAgcauCAGCG-CAGCUACGaaacgaCCGUGCa -3' miRNA: 3'- uUGGU----GUCGCaGUUGAUGCg-----GGCGCG- -5' |
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| 8856 | 5' | -56.3 | NC_002484.1 | + | 39033 | 1.08 | 0.000843 |
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Target: 5'- aAACCACAGCGUCAACUACGCCCGCGCc -3' miRNA: 3'- -UUGGUGUCGCAGUUGAUGCGGGCGCG- -5' |
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| 8856 | 5' | -56.3 | NC_002484.1 | + | 29780 | 1.08 | 0.000843 |
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Target: 5'- aAACCACAGCGUCAACUACGCCCGCGCc -3' miRNA: 3'- -UUGGUGUCGCAGUUGAUGCGGGCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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