Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8858 | 5' | -53.7 | NC_002484.1 | + | 38397 | 0.66 | 0.82113 |
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Target: 5'- cAGCACGCu----ACCGGUGGcugcgggauAGCUGCa -3' miRNA: 3'- -UCGUGCGcauucUGGUCACC---------UUGGCG- -5' |
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| 8858 | 5' | -53.7 | NC_002484.1 | + | 18352 | 0.66 | 0.817398 |
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Target: 5'- cGCugGCGaguucagcaguGACCAGUcGAGCgGCu -3' miRNA: 3'- uCGugCGCauu--------CUGGUCAcCUUGgCG- -5' |
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| 8858 | 5' | -53.7 | NC_002484.1 | + | 47886 | 0.66 | 0.807931 |
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Target: 5'- cGGCACGCGagcagaucaccucGAGACgCGGaaccacguUGGcGCCGCg -3' miRNA: 3'- -UCGUGCGCa------------UUCUG-GUC--------ACCuUGGCG- -5' |
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| 8858 | 5' | -53.7 | NC_002484.1 | + | 24241 | 0.67 | 0.730653 |
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Target: 5'- uGGCAUaGCucuUAAGACCAuGUGGAuugcuucauCCGCa -3' miRNA: 3'- -UCGUG-CGc--AUUCUGGU-CACCUu--------GGCG- -5' |
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| 8858 | 5' | -53.7 | NC_002484.1 | + | 53886 | 0.69 | 0.643136 |
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Target: 5'- cGCAUGCGUGAuuuggaguGACCGGUGcuGAAuCCGg -3' miRNA: 3'- uCGUGCGCAUU--------CUGGUCAC--CUU-GGCg -5' |
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| 8858 | 5' | -53.7 | NC_002484.1 | + | 33525 | 0.69 | 0.620936 |
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Target: 5'- aGGCAgGCG-GGGAUCGGUGGGA-UGCc -3' miRNA: 3'- -UCGUgCGCaUUCUGGUCACCUUgGCG- -5' |
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| 8858 | 5' | -53.7 | NC_002484.1 | + | 38194 | 0.73 | 0.421167 |
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Target: 5'- uGC-CGCGcGAGACCcuGGUGGugaaccuGCCGCg -3' miRNA: 3'- uCGuGCGCaUUCUGG--UCACCu------UGGCG- -5' |
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| 8858 | 5' | -53.7 | NC_002484.1 | + | 8536 | 0.77 | 0.245337 |
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Target: 5'- uGGUGCaGCGgcAGACCuGUcgGGAACCGCg -3' miRNA: 3'- -UCGUG-CGCauUCUGGuCA--CCUUGGCG- -5' |
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| 8858 | 5' | -53.7 | NC_002484.1 | + | 40322 | 0.99 | 0.007501 |
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Target: 5'- cAGCACGCGauAGACCAGUGGAACCGCc -3' miRNA: 3'- -UCGUGCGCauUCUGGUCACCUUGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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