Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8862 | 5' | -61.5 | NC_002484.1 | + | 13227 | 0.66 | 0.394344 |
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Target: 5'- cGUCGGCaagacucuCGCCGUCCAUGaucauCGACGCa-- -3' miRNA: 3'- -CGGCUG--------GCGGCAGGUAC-----GCUGCGgga -5' |
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| 8862 | 5' | -61.5 | NC_002484.1 | + | 13466 | 0.67 | 0.377305 |
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Target: 5'- aGCCG-CCGCCGgggCCAacaGCGG-GCCUa -3' miRNA: 3'- -CGGCuGGCGGCa--GGUa--CGCUgCGGGa -5' |
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| 8862 | 5' | -61.5 | NC_002484.1 | + | 5791 | 0.67 | 0.377305 |
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Target: 5'- -gCGACCGCCGUCCAaguaugaGCGAacugGCUUg -3' miRNA: 3'- cgGCUGGCGGCAGGUa------CGCUg---CGGGa -5' |
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| 8862 | 5' | -61.5 | NC_002484.1 | + | 51164 | 0.67 | 0.352693 |
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Target: 5'- aGCUG--UGUCGUCCAUGCu-CGCCCa -3' miRNA: 3'- -CGGCugGCGGCAGGUACGcuGCGGGa -5' |
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| 8862 | 5' | -61.5 | NC_002484.1 | + | 42624 | 0.68 | 0.320932 |
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Target: 5'- uGCUGGCCuGUCGUUCAgcggauagauaauUGCGAguucCGCCCg -3' miRNA: 3'- -CGGCUGG-CGGCAGGU-------------ACGCU----GCGGGa -5' |
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| 8862 | 5' | -61.5 | NC_002484.1 | + | 36917 | 0.68 | 0.29979 |
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Target: 5'- cGCCGAaCGCCGagcCCAggaagaacGCGACGCCa- -3' miRNA: 3'- -CGGCUgGCGGCa--GGUa-------CGCUGCGGga -5' |
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| 8862 | 5' | -61.5 | NC_002484.1 | + | 19313 | 0.7 | 0.229401 |
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Target: 5'- -gUGGCCGCCuGgcgcaugCaCAUGCGGCGCCCc -3' miRNA: 3'- cgGCUGGCGG-Ca------G-GUACGCUGCGGGa -5' |
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| 8862 | 5' | -61.5 | NC_002484.1 | + | 5142 | 0.71 | 0.212792 |
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Target: 5'- gGCCGAagCGUCGUCCAggaucugGCGACuuGCCUUg -3' miRNA: 3'- -CGGCUg-GCGGCAGGUa------CGCUG--CGGGA- -5' |
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| 8862 | 5' | -61.5 | NC_002484.1 | + | 17331 | 0.86 | 0.015452 |
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Target: 5'- uGCUGACCGUCGUCCAUGCGcCGCCg- -3' miRNA: 3'- -CGGCUGGCGGCAGGUACGCuGCGGga -5' |
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| 8862 | 5' | -61.5 | NC_002484.1 | + | 43134 | 1.09 | 0.000298 |
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Target: 5'- gGCCGACCGCCGUCCAUGCGACGCCCUg -3' miRNA: 3'- -CGGCUGGCGGCAGGUACGCUGCGGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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