Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8865 | 3' | -51.4 | NC_002484.1 | + | 28607 | 0.73 | 0.481004 |
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Target: 5'- cGgGCGCCAcuagcucucccugAGCUAACCC-GGCUgGGCu -3' miRNA: 3'- -CgUGCGGU-------------UUGAUUGGGuCCGAgUUG- -5' |
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| 8865 | 3' | -51.4 | NC_002484.1 | + | 4213 | 0.72 | 0.580893 |
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Target: 5'- uCGCGCCc-GCcGAUCCAGGuCUCGGCg -3' miRNA: 3'- cGUGCGGuuUGaUUGGGUCC-GAGUUG- -5' |
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| 8865 | 3' | -51.4 | NC_002484.1 | + | 52403 | 0.69 | 0.727813 |
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Target: 5'- aGCugGCCGAuGCcgGACagcagggaaAGGCUCAGCg -3' miRNA: 3'- -CGugCGGUU-UGa-UUGgg-------UCCGAGUUG- -5' |
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| 8865 | 3' | -51.4 | NC_002484.1 | + | 6640 | 0.69 | 0.727813 |
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Target: 5'- cCACGCUAGGCggaAugCCuGGCUCGcuGCu -3' miRNA: 3'- cGUGCGGUUUGa--UugGGuCCGAGU--UG- -5' |
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| 8865 | 3' | -51.4 | NC_002484.1 | + | 51800 | 0.69 | 0.73874 |
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Target: 5'- cGCAgGCCGGggagcacacgcGCUGcCCCAGGCgcuGCg -3' miRNA: 3'- -CGUgCGGUU-----------UGAUuGGGUCCGaguUG- -5' |
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| 8865 | 3' | -51.4 | NC_002484.1 | + | 40162 | 0.66 | 0.881983 |
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Target: 5'- --cCGCCAccCUAgGCCCGGGCUgAAa -3' miRNA: 3'- cguGCGGUuuGAU-UGGGUCCGAgUUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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