Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8866 | 3' | -56.9 | NC_002484.1 | + | 16731 | 0.66 | 0.647718 |
Target: 5'- aGUCaGaGGGGAACGaCCCGGCU--CCGg -3' miRNA: 3'- aCAGcC-CUCUUUGC-GGGUCGAguGGC- -5' |
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8866 | 3' | -56.9 | NC_002484.1 | + | 14820 | 0.66 | 0.636863 |
Target: 5'- -uUCGGGAGcGAC-CCUAGCcaCACCa -3' miRNA: 3'- acAGCCCUCuUUGcGGGUCGa-GUGGc -5' |
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8866 | 3' | -56.9 | NC_002484.1 | + | 47978 | 0.66 | 0.604311 |
Target: 5'- gGUgCGGcuguuuGAGcauGGCGCCCAGCaCGCCGu -3' miRNA: 3'- aCA-GCC------CUCu--UUGCGGGUCGaGUGGC- -5' |
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8866 | 3' | -56.9 | NC_002484.1 | + | 46771 | 0.7 | 0.401741 |
Target: 5'- cUGUUGGuacuccucucGAGAAGCGgCCGGCUCuCCa -3' miRNA: 3'- -ACAGCC----------CUCUUUGCgGGUCGAGuGGc -5' |
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8866 | 3' | -56.9 | NC_002484.1 | + | 47222 | 1.08 | 0.0009 |
Target: 5'- cUGUCGGGAGAAACGCCCAGCUCACCGc -3' miRNA: 3'- -ACAGCCCUCUUUGCGGGUCGAGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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