Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8869 | 5' | -51.4 | NC_002484.1 | + | 47199 | 0.66 | 0.905915 |
Target: 5'- aCCGcgGCUUCCcccgAUGCG-GAacaggaugcgccucUCCAGCAg -3' miRNA: 3'- -GGUuaUGAGGG----UACGCuCU--------------AGGUCGU- -5' |
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8869 | 5' | -51.4 | NC_002484.1 | + | 53093 | 0.68 | 0.797885 |
Target: 5'- cCCucgACUCCCuucaacUGCGGGAUCgcccaGGCAg -3' miRNA: 3'- -GGuuaUGAGGGu-----ACGCUCUAGg----UCGU- -5' |
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8869 | 5' | -51.4 | NC_002484.1 | + | 30333 | 0.71 | 0.668857 |
Target: 5'- cCgGAUGCUCUUGUGCcgGAGAacaacguaUCCAGCAa -3' miRNA: 3'- -GgUUAUGAGGGUACG--CUCU--------AGGUCGU- -5' |
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8869 | 5' | -51.4 | NC_002484.1 | + | 50874 | 1.11 | 0.001777 |
Target: 5'- cCCAAUACUCCCAUGCGAGAUCCAGCAu -3' miRNA: 3'- -GGUUAUGAGGGUACGCUCUAGGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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