Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8870 | 5' | -51.9 | NC_002484.1 | + | 41669 | 0.66 | 0.86178 |
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Target: 5'- gCcgCGCCcGGCaaGGAGgGGGUGUGAUg -3' miRNA: 3'- aGuaGCGGaCCG--UCUUgUCCGUAUUG- -5' |
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| 8870 | 5' | -51.9 | NC_002484.1 | + | 48984 | 0.66 | 0.852277 |
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Target: 5'- aCAUcCGCgguugagCUGGCAGAGCGGGagcaccgGGCa -3' miRNA: 3'- aGUA-GCG-------GACCGUCUUGUCCgua----UUG- -5' |
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| 8870 | 5' | -51.9 | NC_002484.1 | + | 27782 | 0.67 | 0.825814 |
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Target: 5'- ---gCGCgaGGCAGAG-GGGCGUGAUc -3' miRNA: 3'- aguaGCGgaCCGUCUUgUCCGUAUUG- -5' |
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| 8870 | 5' | -51.9 | NC_002484.1 | + | 32426 | 0.67 | 0.825814 |
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Target: 5'- ----gGCCUGaucaaGCAGAACAGGCGc--- -3' miRNA: 3'- aguagCGGAC-----CGUCUUGUCCGUauug -5' |
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| 8870 | 5' | -51.9 | NC_002484.1 | + | 599 | 0.67 | 0.800512 |
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Target: 5'- gUCAUCGCuCUGGCgguccaagucauucaGGAagcGCuGGCAUGcaaGCg -3' miRNA: 3'- -AGUAGCG-GACCG---------------UCU---UGuCCGUAU---UG- -5' |
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| 8870 | 5' | -51.9 | NC_002484.1 | + | 14862 | 0.68 | 0.776013 |
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Target: 5'- cUAUCGCCuuugagaugUGGCAacGcGCAGGCGUuACg -3' miRNA: 3'- aGUAGCGG---------ACCGU--CuUGUCCGUAuUG- -5' |
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| 8870 | 5' | -51.9 | NC_002484.1 | + | 33543 | 0.68 | 0.733214 |
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Target: 5'- aCAagGCaCcGGUAGAGCAGGCA-GGCg -3' miRNA: 3'- aGUagCG-GaCCGUCUUGUCCGUaUUG- -5' |
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| 8870 | 5' | -51.9 | NC_002484.1 | + | 9037 | 0.71 | 0.552337 |
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Target: 5'- gUAUUGCUUGG-AGGACAGGCAgucAGCc -3' miRNA: 3'- aGUAGCGGACCgUCUUGUCCGUa--UUG- -5' |
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| 8870 | 5' | -51.9 | NC_002484.1 | + | 18060 | 0.72 | 0.530125 |
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Target: 5'- aCAUCGCCgGGCAG-GC-GGCAcugGACg -3' miRNA: 3'- aGUAGCGGaCCGUCuUGuCCGUa--UUG- -5' |
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| 8870 | 5' | -51.9 | NC_002484.1 | + | 51388 | 1.1 | 0.001657 |
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Target: 5'- uUCAUCGCCUGGCAGAACAGGCAUAACc -3' miRNA: 3'- -AGUAGCGGACCGUCUUGUCCGUAUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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