Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8871 | 5' | -51.7 | NC_002484.1 | + | 11403 | 0.66 | 0.879503 |
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Target: 5'- cGGCCaCGAAGCCGcCAA-CCUUg-- -3' miRNA: 3'- cCCGGaGUUUCGGCaGUUaGGAGuuu -5' |
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| 8871 | 5' | -51.7 | NC_002484.1 | + | 37443 | 0.67 | 0.827345 |
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Target: 5'- aGGGCagaGAGGCCGUCg--CCUCc-- -3' miRNA: 3'- -CCCGgagUUUCGGCAGuuaGGAGuuu -5' |
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| 8871 | 5' | -51.7 | NC_002484.1 | + | 55552 | 0.68 | 0.788033 |
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Target: 5'- uGGGCUUUcgGGCCGcUCGAUCUUguAGc -3' miRNA: 3'- -CCCGGAGuuUCGGC-AGUUAGGAguUU- -5' |
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| 8871 | 5' | -51.7 | NC_002484.1 | + | 11796 | 0.7 | 0.645172 |
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Target: 5'- aGGGCCUgGGGGCCGcUCAGauUCCg---- -3' miRNA: 3'- -CCCGGAgUUUCGGC-AGUU--AGGaguuu -5' |
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| 8871 | 5' | -51.7 | NC_002484.1 | + | 17763 | 0.72 | 0.554358 |
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Target: 5'- cGGGCCUucCAGauugcguccAGCCGaUCGAguUCCUCAAGa -3' miRNA: 3'- -CCCGGA--GUU---------UCGGC-AGUU--AGGAGUUU- -5' |
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| 8871 | 5' | -51.7 | NC_002484.1 | + | 18434 | 0.74 | 0.407448 |
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Target: 5'- uGGGaaugCUCAGccgguAGUCGUCGAUCCUCGAc -3' miRNA: 3'- -CCCg---GAGUU-----UCGGCAGUUAGGAGUUu -5' |
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| 8871 | 5' | -51.7 | NC_002484.1 | + | 53372 | 1.09 | 0.002146 |
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Target: 5'- aGGGCCUCAAAGCCGUCAAUCCUCAAAc -3' miRNA: 3'- -CCCGGAGUUUCGGCAGUUAGGAGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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