Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8876 | 3' | -55.5 | NC_002484.1 | + | 13008 | 0.66 | 0.647041 |
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Target: 5'- cAGCgagAGCCUGgCGcAgaaggucaguuucuuGGUCGUAGAGUUCc -3' miRNA: 3'- -UCG---UCGGACgGC-U---------------CCAGCGUUUCAAG- -5' |
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| 8876 | 3' | -55.5 | NC_002484.1 | + | 32895 | 0.67 | 0.598035 |
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Target: 5'- cGCcGCCcugGCCaGGGUCGCGGAGc-- -3' miRNA: 3'- uCGuCGGa--CGGcUCCAGCGUUUCaag -5' |
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| 8876 | 3' | -55.5 | NC_002484.1 | + | 39635 | 0.67 | 0.5759 |
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Target: 5'- aGGCaugAGCCgauccGCUGcGGUCGCcAAGUUCc -3' miRNA: 3'- -UCG---UCGGa----CGGCuCCAGCGuUUCAAG- -5' |
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| 8876 | 3' | -55.5 | NC_002484.1 | + | 51980 | 0.69 | 0.510886 |
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Target: 5'- uGCAGCCgcUGCCGAuGGaaaguUCGU-AGGUUCg -3' miRNA: 3'- uCGUCGG--ACGGCU-CC-----AGCGuUUCAAG- -5' |
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| 8876 | 3' | -55.5 | NC_002484.1 | + | 8528 | 0.69 | 0.510886 |
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Target: 5'- cGGCAGaCCUGUCGGGaacCGCGAAGg-- -3' miRNA: 3'- -UCGUC-GGACGGCUCca-GCGUUUCaag -5' |
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| 8876 | 3' | -55.5 | NC_002484.1 | + | 13137 | 0.71 | 0.359639 |
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Target: 5'- cGGCAGCCUGCUGuuGauaauccguggccguGUCGCGGuAGUUCu -3' miRNA: 3'- -UCGUCGGACGGCu-C---------------CAGCGUU-UCAAG- -5' |
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| 8876 | 3' | -55.5 | NC_002484.1 | + | 1551 | 0.72 | 0.347671 |
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Target: 5'- cGGCAGCCUGCCau-GUCGCAAucGUg- -3' miRNA: 3'- -UCGUCGGACGGcucCAGCGUUu-CAag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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