Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8878 | 5' | -55.1 | NC_002484.1 | + | 2547 | 0.67 | 0.68277 |
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Target: 5'- -aAGCGGAAGaccgGGACaGAAGUAGAGa -3' miRNA: 3'- gcUCGCCUUUcg--CCUGcCUUCGUCUC- -5' |
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| 8878 | 5' | -55.1 | NC_002484.1 | + | 50725 | 0.67 | 0.671874 |
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Target: 5'- uGGGCGGu--GCGGAUGaGAGC-GAGu -3' miRNA: 3'- gCUCGCCuuuCGCCUGCcUUCGuCUC- -5' |
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| 8878 | 5' | -55.1 | NC_002484.1 | + | 35606 | 0.67 | 0.671874 |
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Target: 5'- uGAuUGGuGAGCGGAUcaccGGAGCAGAGg -3' miRNA: 3'- gCUcGCCuUUCGCCUGc---CUUCGUCUC- -5' |
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| 8878 | 5' | -55.1 | NC_002484.1 | + | 52629 | 0.67 | 0.648884 |
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Target: 5'- --uGCGGAAGGCGGugGagacgaucagGAacugcaccaugaaGGCGGAGg -3' miRNA: 3'- gcuCGCCUUUCGCCugC----------CU-------------UCGUCUC- -5' |
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| 8878 | 5' | -55.1 | NC_002484.1 | + | 7202 | 0.7 | 0.498997 |
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Target: 5'- aCGcAGCGG--GGCGGACGGAugAGCGa-- -3' miRNA: 3'- -GC-UCGCCuuUCGCCUGCCU--UCGUcuc -5' |
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| 8878 | 5' | -55.1 | NC_002484.1 | + | 33150 | 0.71 | 0.429246 |
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Target: 5'- aGAGCaGGcGGAGCGGcCGGAGGUGGuGg -3' miRNA: 3'- gCUCG-CC-UUUCGCCuGCCUUCGUCuC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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