Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8879 | 5' | -55.9 | NC_002484.1 | + | 31509 | 0.66 | 0.688028 |
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Target: 5'- --cGCAGGCCAuguggUGGUCaGCGagGAucUGCu -3' miRNA: 3'- gacCGUCCGGU-----ACCAG-CGUagCU--ACG- -5' |
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| 8879 | 5' | -55.9 | NC_002484.1 | + | 2653 | 0.66 | 0.644224 |
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Target: 5'- aCUGGaAGGCCGUcauGUCGuCGUUGggGCu -3' miRNA: 3'- -GACCgUCCGGUAc--CAGC-GUAGCuaCG- -5' |
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| 8879 | 5' | -55.9 | NC_002484.1 | + | 39371 | 0.68 | 0.555604 |
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Target: 5'- gCUGGUGucacguuugcaauGGCCGUGG-CGuCGUUGAUGUc -3' miRNA: 3'- -GACCGU-------------CCGGUACCaGC-GUAGCUACG- -5' |
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| 8879 | 5' | -55.9 | NC_002484.1 | + | 5478 | 0.68 | 0.545924 |
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Target: 5'- -cGGCAGacGCCAgagGGUCGgAgUGGUGCc -3' miRNA: 3'- gaCCGUC--CGGUa--CCAGCgUaGCUACG- -5' |
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| 8879 | 5' | -55.9 | NC_002484.1 | + | 6773 | 0.72 | 0.352918 |
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Target: 5'- -cGGUAGGCCAgcauucaaacccccaGGcCGCAUCGAUacgGCa -3' miRNA: 3'- gaCCGUCCGGUa--------------CCaGCGUAGCUA---CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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