Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8881 | 3' | -55.1 | NC_002484.1 | + | 918 | 0.66 | 0.782141 |
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Target: 5'- ---aGGCCUGCuucuCGguGGUggCGCCGGa -3' miRNA: 3'- aguaCUGGGCGuu--GCuuCCA--GCGGCC- -5' |
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| 8881 | 3' | -55.1 | NC_002484.1 | + | 33440 | 0.66 | 0.77226 |
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Target: 5'- --cUGAUCCGCAACGcAGcucgCGCCGc -3' miRNA: 3'- aguACUGGGCGUUGCuUCca--GCGGCc -5' |
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| 8881 | 3' | -55.1 | NC_002484.1 | + | 49035 | 0.66 | 0.762237 |
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Target: 5'- gCcgGACUCGCGu---AGGUCGCUGa -3' miRNA: 3'- aGuaCUGGGCGUugcuUCCAGCGGCc -5' |
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| 8881 | 3' | -55.1 | NC_002484.1 | + | 48779 | 0.66 | 0.741813 |
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Target: 5'- gUCAUGACCgGCAcgucgcucacgGCGAucuuGGcGUCGCgGa -3' miRNA: 3'- -AGUACUGGgCGU-----------UGCU----UC-CAGCGgCc -5' |
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| 8881 | 3' | -55.1 | NC_002484.1 | + | 30018 | 0.66 | 0.7283 |
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Target: 5'- gCAUGACCgaccaCGCAgagcuGCgGAGGGUggcugaggcggcaaCGCCGGg -3' miRNA: 3'- aGUACUGG-----GCGU-----UG-CUUCCA--------------GCGGCC- -5' |
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| 8881 | 3' | -55.1 | NC_002484.1 | + | 31170 | 0.7 | 0.527631 |
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Target: 5'- ---cGGCUucacucugCGCAACGGAGGUCaCCGGu -3' miRNA: 3'- aguaCUGG--------GCGUUGCUUCCAGcGGCC- -5' |
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| 8881 | 3' | -55.1 | NC_002484.1 | + | 14745 | 0.72 | 0.417441 |
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Target: 5'- -gAUGugCCGCAGCGcggcgguauugauGAGGUCguaguaGCCGGc -3' miRNA: 3'- agUACugGGCGUUGC-------------UUCCAG------CGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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