Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8882 | 3' | -51.6 | NC_002484.1 | + | 9188 | 0.66 | 0.893171 |
Target: 5'- aGcCAUGGacucucCGGAACGCAaaGCUGGUGa -3' miRNA: 3'- aCaGUGCC------GUUUUGCGUaaCGACCGC- -5' |
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8882 | 3' | -51.6 | NC_002484.1 | + | 8222 | 0.66 | 0.893171 |
Target: 5'- ---uGCGGCGcaucucuGAgGCAgcGCUGGCGa -3' miRNA: 3'- acagUGCCGUu------UUgCGUaaCGACCGC- -5' |
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8882 | 3' | -51.6 | NC_002484.1 | + | 51738 | 0.67 | 0.843964 |
Target: 5'- gUGUUcUGGCAcuucuuGGgGCGggGCUGGCGg -3' miRNA: 3'- -ACAGuGCCGUu-----UUgCGUaaCGACCGC- -5' |
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8882 | 3' | -51.6 | NC_002484.1 | + | 27360 | 0.68 | 0.765528 |
Target: 5'- cUG-CGCGGCAugGCuggucagcgccgGCGUUccGCUGGCGg -3' miRNA: 3'- -ACaGUGCCGUuuUG------------CGUAA--CGACCGC- -5' |
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8882 | 3' | -51.6 | NC_002484.1 | + | 16060 | 0.7 | 0.655105 |
Target: 5'- gGUCACcGCAGAGCGCAac-CUcGGCGg -3' miRNA: 3'- aCAGUGcCGUUUUGCGUaacGA-CCGC- -5' |
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8882 | 3' | -51.6 | NC_002484.1 | + | 18374 | 0.73 | 0.498601 |
Target: 5'- cGUCugGGCGc--CGCAgguagagcGCUGGCGa -3' miRNA: 3'- aCAGugCCGUuuuGCGUaa------CGACCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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