Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8887 | 3' | -59.8 | NC_002484.1 | + | 3669 | 0.67 | 0.446482 |
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Target: 5'- aGCGCGGGCgaACcuccgGCGCUGCCGGa--- -3' miRNA: 3'- -CGUGUCCGgaUG-----CGCGGCGGCUcacc -5' |
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| 8887 | 3' | -59.8 | NC_002484.1 | + | 25260 | 0.67 | 0.427857 |
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Target: 5'- cGCACGGGCaca---GCCGUCGAG-GGu -3' miRNA: 3'- -CGUGUCCGgaugcgCGGCGGCUCaCC- -5' |
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| 8887 | 3' | -59.8 | NC_002484.1 | + | 7161 | 0.68 | 0.383382 |
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Target: 5'- uGUACGGGCCUagcuGCGUGUuaggUGCCcuuGUGGg -3' miRNA: 3'- -CGUGUCCGGA----UGCGCG----GCGGcu-CACC- -5' |
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| 8887 | 3' | -59.8 | NC_002484.1 | + | 5867 | 0.69 | 0.350083 |
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Target: 5'- gGCACAGGCCU-CGgGCCaccaauuggcgcGCCaGGUGc -3' miRNA: 3'- -CGUGUCCGGAuGCgCGG------------CGGcUCACc -5' |
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| 8887 | 3' | -59.8 | NC_002484.1 | + | 15775 | 0.69 | 0.342086 |
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Target: 5'- -aGCAGGCCUACGUgaucgagcaGCCGCCcucuGUaGGc -3' miRNA: 3'- cgUGUCCGGAUGCG---------CGGCGGcu--CA-CC- -5' |
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| 8887 | 3' | -59.8 | NC_002484.1 | + | 14839 | 0.7 | 0.304099 |
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Target: 5'- cGCGCAGGCgUUACGCGCUuUCGGGa-- -3' miRNA: 3'- -CGUGUCCG-GAUGCGCGGcGGCUCacc -5' |
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| 8887 | 3' | -59.8 | NC_002484.1 | + | 51802 | 0.7 | 0.289838 |
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Target: 5'- gGCGCAGGCCggggagcacACGCGCUGCCcca-GGc -3' miRNA: 3'- -CGUGUCCGGa--------UGCGCGGCGGcucaCC- -5' |
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| 8887 | 3' | -59.8 | NC_002484.1 | + | 42285 | 0.71 | 0.250211 |
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Target: 5'- cGUGC--GCCUGCGCGCUGCUGAGg-- -3' miRNA: 3'- -CGUGucCGGAUGCGCGGCGGCUCacc -5' |
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| 8887 | 3' | -59.8 | NC_002484.1 | + | 3911 | 0.75 | 0.141739 |
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Target: 5'- gGC-CAGGCCUucgaauACGuCGCCGCCGGGg-- -3' miRNA: 3'- -CGuGUCCGGA------UGC-GCGGCGGCUCacc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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