Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8888 | 5' | -61.6 | NC_002484.1 | + | 15707 | 0.66 | 0.409049 |
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Target: 5'- cUGCUCAagcgcgcgcuggaucUCCGUaUCGCacgCCCGCUGCa- -3' miRNA: 3'- aGCGAGU---------------AGGCA-GGCG---GGGCGACGca -5' |
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| 8888 | 5' | -61.6 | NC_002484.1 | + | 26211 | 0.66 | 0.396708 |
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Target: 5'- gCGCggcaAUCUGcCCGCCCUGCUccacuacuGCGg -3' miRNA: 3'- aGCGag--UAGGCaGGCGGGGCGA--------CGCa -5' |
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| 8888 | 5' | -61.6 | NC_002484.1 | + | 46935 | 0.66 | 0.38718 |
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Target: 5'- aUUGCUCAuacucugUCCGUCCGUucgugauguUCC-CUGCGUu -3' miRNA: 3'- -AGCGAGU-------AGGCAGGCG---------GGGcGACGCA- -5' |
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| 8888 | 5' | -61.6 | NC_002484.1 | + | 44215 | 0.67 | 0.371083 |
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Target: 5'- aCGUgCGUCCuccguGUCCGCCCCGUccGCa- -3' miRNA: 3'- aGCGaGUAGG-----CAGGCGGGGCGa-CGca -5' |
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| 8888 | 5' | -61.6 | NC_002484.1 | + | 2586 | 0.7 | 0.22445 |
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Target: 5'- aUCGaCUCugcuUCCGUCCGCUuuCCGCU-CGUa -3' miRNA: 3'- -AGC-GAGu---AGGCAGGCGG--GGCGAcGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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