miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
8888 5' -61.6 NC_002484.1 + 15707 0.66 0.409049
Target:  5'- cUGCUCAagcgcgcgcuggaucUCCGUaUCGCacgCCCGCUGCa- -3'
miRNA:   3'- aGCGAGU---------------AGGCA-GGCG---GGGCGACGca -5'
8888 5' -61.6 NC_002484.1 + 26211 0.66 0.396708
Target:  5'- gCGCggcaAUCUGcCCGCCCUGCUccacuacuGCGg -3'
miRNA:   3'- aGCGag--UAGGCaGGCGGGGCGA--------CGCa -5'
8888 5' -61.6 NC_002484.1 + 46935 0.66 0.38718
Target:  5'- aUUGCUCAuacucugUCCGUCCGUucgugauguUCC-CUGCGUu -3'
miRNA:   3'- -AGCGAGU-------AGGCAGGCG---------GGGcGACGCA- -5'
8888 5' -61.6 NC_002484.1 + 44215 0.67 0.371083
Target:  5'- aCGUgCGUCCuccguGUCCGCCCCGUccGCa- -3'
miRNA:   3'- aGCGaGUAGG-----CAGGCGGGGCGa-CGca -5'
8888 5' -61.6 NC_002484.1 + 2586 0.7 0.22445
Target:  5'- aUCGaCUCugcuUCCGUCCGCUuuCCGCU-CGUa -3'
miRNA:   3'- -AGC-GAGu---AGGCAGGCGG--GGCGAcGCA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.