Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8889 | 5' | -59.4 | NC_002484.1 | + | 37894 | 0.66 | 0.498003 |
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Target: 5'- cUCgCAUUaaUGCCGGCAGGCGgcugCCAGc -3' miRNA: 3'- -AG-GUGAggGCGGCCGUCUGUa---GGUCa -5' |
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| 8889 | 5' | -59.4 | NC_002484.1 | + | 28662 | 0.66 | 0.495985 |
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Target: 5'- cCCACUCUaCGCUGGCgAGACAguagaucaagaaCCGGa -3' miRNA: 3'- aGGUGAGG-GCGGCCG-UCUGUa-----------GGUCa -5' |
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| 8889 | 5' | -59.4 | NC_002484.1 | + | 26591 | 0.66 | 0.47207 |
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Target: 5'- aUCCGCUgCCGCCGcucggccagcagcgcGCGGACcuugGUCUGGa -3' miRNA: 3'- -AGGUGAgGGCGGC---------------CGUCUG----UAGGUCa -5' |
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| 8889 | 5' | -59.4 | NC_002484.1 | + | 11439 | 0.67 | 0.429816 |
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Target: 5'- gCCACUuuUCCGaCCGGCGcucggcGGCAUCCAu- -3' miRNA: 3'- aGGUGA--GGGC-GGCCGU------CUGUAGGUca -5' |
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| 8889 | 5' | -59.4 | NC_002484.1 | + | 48084 | 0.67 | 0.429816 |
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Target: 5'- aUCgGCg--UGCCGGCAGAC-UCCAGa -3' miRNA: 3'- -AGgUGaggGCGGCCGUCUGuAGGUCa -5' |
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| 8889 | 5' | -59.4 | NC_002484.1 | + | 26174 | 0.67 | 0.420525 |
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Target: 5'- cUCCagGCUCCCacgaGCggaaCGGCAGGCAgCCAGa -3' miRNA: 3'- -AGG--UGAGGG----CG----GCCGUCUGUaGGUCa -5' |
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| 8889 | 5' | -59.4 | NC_002484.1 | + | 13875 | 0.68 | 0.359043 |
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Target: 5'- gCC-CUCCCGCUgaggcgGGCAGAUAUuuGGg -3' miRNA: 3'- aGGuGAGGGCGG------CCGUCUGUAggUCa -5' |
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| 8889 | 5' | -59.4 | NC_002484.1 | + | 27337 | 0.7 | 0.289671 |
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Target: 5'- cCgGCgUUCCGCUGGCGGAUGUUCGGg -3' miRNA: 3'- aGgUG-AGGGCGGCCGUCUGUAGGUCa -5' |
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| 8889 | 5' | -59.4 | NC_002484.1 | + | 43531 | 0.73 | 0.16913 |
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Target: 5'- aCCACcgCuuGCCuGGCuGGCAUCCAGg -3' miRNA: 3'- aGGUGa-GggCGG-CCGuCUGUAGGUCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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