Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
889 | 3' | -53.4 | NC_000899.1 | + | 1345 | 0.66 | 0.772358 |
Target: 5'- aCCGCCGguggagccgaaaccGGCUGCACcccuGGcCGUcucgucuaAGCCc -3' miRNA: 3'- aGGCGGC--------------UCGACGUGu---UCaGCA--------UUGG- -5' |
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889 | 3' | -53.4 | NC_000899.1 | + | 19925 | 0.66 | 0.754587 |
Target: 5'- aUCCGCCGucGGCUGcCGCuauGGccgCGUuuacACCg -3' miRNA: 3'- -AGGCGGC--UCGAC-GUGu--UCa--GCAu---UGG- -5' |
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889 | 3' | -53.4 | NC_000899.1 | + | 9579 | 0.67 | 0.733198 |
Target: 5'- -aUGCCGAGUUcgucuuugaaGUACuuGUCGUAACUg -3' miRNA: 3'- agGCGGCUCGA----------CGUGuuCAGCAUUGG- -5' |
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889 | 3' | -53.4 | NC_000899.1 | + | 15196 | 0.67 | 0.722337 |
Target: 5'- aCUGCCGGGCUcCAUAGcccCGUAAUCg -3' miRNA: 3'- aGGCGGCUCGAcGUGUUca-GCAUUGG- -5' |
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889 | 3' | -53.4 | NC_000899.1 | + | 7135 | 0.67 | 0.700347 |
Target: 5'- cUCCcCCGAGaCUGUAaAAGUCGUcgaAGCUg -3' miRNA: 3'- -AGGcGGCUC-GACGUgUUCAGCA---UUGG- -5' |
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889 | 3' | -53.4 | NC_000899.1 | + | 13961 | 0.67 | 0.693691 |
Target: 5'- cCCGUCGGGaugaucgacguacguCUGCACgAAGUCGUAc-- -3' miRNA: 3'- aGGCGGCUC---------------GACGUG-UUCAGCAUugg -5' |
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889 | 3' | -53.4 | NC_000899.1 | + | 10444 | 0.68 | 0.63308 |
Target: 5'- uUCCGggucaaCGAGC-GC-CAGGUCcGUGGCCg -3' miRNA: 3'- -AGGCg-----GCUCGaCGuGUUCAG-CAUUGG- -5' |
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889 | 3' | -53.4 | NC_000899.1 | + | 12998 | 0.69 | 0.610532 |
Target: 5'- gUCC-CUGAGCUGCAUcaaucuguAGUCGUAGa- -3' miRNA: 3'- -AGGcGGCUCGACGUGu-------UCAGCAUUgg -5' |
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889 | 3' | -53.4 | NC_000899.1 | + | 16877 | 0.7 | 0.55467 |
Target: 5'- cUCCaGCCGGuGCUcaGUACAAGUgGUacGACCu -3' miRNA: 3'- -AGG-CGGCU-CGA--CGUGUUCAgCA--UUGG- -5' |
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889 | 3' | -53.4 | NC_000899.1 | + | 15573 | 0.7 | 0.532717 |
Target: 5'- cCCGCCGGGCUcuaCACGGGUaacuaCGUcauACCg -3' miRNA: 3'- aGGCGGCUCGAc--GUGUUCA-----GCAu--UGG- -5' |
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889 | 3' | -53.4 | NC_000899.1 | + | 10624 | 0.71 | 0.47935 |
Target: 5'- -aCGCaCGuGUUGcCACAGGUCGcAGCCg -3' miRNA: 3'- agGCG-GCuCGAC-GUGUUCAGCaUUGG- -5' |
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889 | 3' | -53.4 | NC_000899.1 | + | 29574 | 0.71 | 0.448612 |
Target: 5'- gCCGCCugaGAGCUGCAUAcGccccUCGgcGCCu -3' miRNA: 3'- aGGCGG---CUCGACGUGUuC----AGCauUGG- -5' |
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889 | 3' | -53.4 | NC_000899.1 | + | 1022 | 1.14 | 0.0006 |
Target: 5'- gUCCGCCGAGCUGCACAAGUCGUAACCg -3' miRNA: 3'- -AGGCGGCUCGACGUGUUCAGCAUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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