Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8895 | 3' | -57.2 | NC_002484.1 | + | 38377 | 0.66 | 0.613071 |
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Target: 5'- cGGGAuAGCUGCaCCcGGCaauacgacgacauGcGCCGCc -3' miRNA: 3'- -CCCUuUCGACG-GGuCCGau-----------CaCGGCG- -5' |
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| 8895 | 3' | -57.2 | NC_002484.1 | + | 12204 | 0.66 | 0.597811 |
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Target: 5'- uGGAAGGCU-CCaacaguGGCUAccGCCGCu -3' miRNA: 3'- cCCUUUCGAcGGgu----CCGAUcaCGGCG- -5' |
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| 8895 | 3' | -57.2 | NC_002484.1 | + | 8941 | 0.69 | 0.426644 |
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Target: 5'- cGGGAugugcuccAGGCUGuCCCAGcaggucacggguucuGCgUGGUGCCGg -3' miRNA: 3'- -CCCU--------UUCGAC-GGGUC---------------CG-AUCACGGCg -5' |
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| 8895 | 3' | -57.2 | NC_002484.1 | + | 38043 | 0.7 | 0.343425 |
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Target: 5'- uGGAuGGCUGCCCAaaGUUGGUGaCCGa -3' miRNA: 3'- cCCUuUCGACGGGUc-CGAUCAC-GGCg -5' |
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| 8895 | 3' | -57.2 | NC_002484.1 | + | 27924 | 0.71 | 0.311735 |
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Target: 5'- gGGGAuacgGAGC-GCaCAGGCUGGaUGCCGg -3' miRNA: 3'- -CCCU----UUCGaCGgGUCCGAUC-ACGGCg -5' |
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| 8895 | 3' | -57.2 | NC_002484.1 | + | 33049 | 0.71 | 0.304167 |
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Target: 5'- cGGAagaucAAGCUGCCCAcuGGCgugAGcgcgGCUGCg -3' miRNA: 3'- cCCU-----UUCGACGGGU--CCGa--UCa---CGGCG- -5' |
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| 8895 | 3' | -57.2 | NC_002484.1 | + | 51560 | 0.73 | 0.230146 |
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Target: 5'- -----uGCUGCUCGGGCUGGaagucGCCGCg -3' miRNA: 3'- cccuuuCGACGGGUCCGAUCa----CGGCG- -5' |
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| 8895 | 3' | -57.2 | NC_002484.1 | + | 51793 | 0.75 | 0.185358 |
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Target: 5'- cGGGGAGcacacgcGCUGcCCCAGGCgcUgcggaacgaacugGGUGCCGCa -3' miRNA: 3'- -CCCUUU-------CGAC-GGGUCCG--A-------------UCACGGCG- -5' |
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| 8895 | 3' | -57.2 | NC_002484.1 | + | 36464 | 0.76 | 0.142064 |
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Target: 5'- cGGGGGAGuCUGCCaccaucuugCAGGUUAuccagcGUGCCGCa -3' miRNA: 3'- -CCCUUUC-GACGG---------GUCCGAU------CACGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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