Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8896 | 5' | -65.1 | NC_002484.1 | + | 40427 | 0.66 | 0.252587 |
Target: 5'- uGCCCCcuguguGGCGGC-GCUGCUuucgUCGggccGCUGa -3' miRNA: 3'- cCGGGG------CCGCCGcCGACGA----AGC----CGAC- -5' |
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8896 | 5' | -65.1 | NC_002484.1 | + | 51877 | 0.67 | 0.234789 |
Target: 5'- cGCCuuGaucuccCGGCGGUUGCggUCGGCg- -3' miRNA: 3'- cCGGggCc-----GCCGCCGACGa-AGCCGac -5' |
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8896 | 5' | -65.1 | NC_002484.1 | + | 26354 | 0.68 | 0.19734 |
Target: 5'- cGCCUCGGCGGCcGCacacUGCUcacCGGCg- -3' miRNA: 3'- cCGGGGCCGCCGcCG----ACGAa--GCCGac -5' |
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8896 | 5' | -65.1 | NC_002484.1 | + | 34111 | 0.68 | 0.18295 |
Target: 5'- cGCCCUGGCGGUGuGacgGUcacCGGCUGg -3' miRNA: 3'- cCGGGGCCGCCGC-Cga-CGaa-GCCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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