Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8897 | 5' | -51.8 | NC_002484.1 | + | 4876 | 0.66 | 0.891561 |
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Target: 5'- cGCCGCCAGAgcCGUCGAUGgagGUGa -3' miRNA: 3'- aCGGCGGUCUaaGUGGCUGUagaUAC- -5' |
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| 8897 | 5' | -51.8 | NC_002484.1 | + | 38231 | 0.66 | 0.868055 |
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Target: 5'- aUGCCgGCCGG---CGCCGAUAUCg--- -3' miRNA: 3'- -ACGG-CGGUCuaaGUGGCUGUAGauac -5' |
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| 8897 | 5' | -51.8 | NC_002484.1 | + | 29044 | 0.67 | 0.814264 |
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Target: 5'- cGCCGCC-GAg--AUCGGCAagUCUAUGg -3' miRNA: 3'- aCGGCGGuCUaagUGGCUGU--AGAUAC- -5' |
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| 8897 | 5' | -51.8 | NC_002484.1 | + | 3891 | 0.68 | 0.7946 |
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Target: 5'- cGCCGCCGGGgcuguugaugUUCACggucacauCGACAUCguugccgAUGg -3' miRNA: 3'- aCGGCGGUCU----------AAGUG--------GCUGUAGa------UAC- -5' |
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| 8897 | 5' | -51.8 | NC_002484.1 | + | 16794 | 0.69 | 0.72075 |
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Target: 5'- cGCCGCCAucGUUCACCGAauagggguaCAUCg--- -3' miRNA: 3'- aCGGCGGUc-UAAGUGGCU---------GUAGauac -5' |
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| 8897 | 5' | -51.8 | NC_002484.1 | + | 29581 | 0.74 | 0.435847 |
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Target: 5'- gUGCUGCgCAGGUUCGCCGGCGa----- -3' miRNA: 3'- -ACGGCG-GUCUAAGUGGCUGUagauac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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