miRNA display CGI


Results 1 - 10 of 10 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
8898 5' -51.3 NC_002484.1 + 1453 0.66 0.920402
Target:  5'- uGCUUGGUGcggaaGGCGUUcuGCUUCGACGc -3'
miRNA:   3'- -UGGACCACuug--CUGUAG--UGGAGUUGC- -5'
8898 5' -51.3 NC_002484.1 + 11950 0.66 0.90748
Target:  5'- gGCCUGG---GCGGCGUCACCa----- -3'
miRNA:   3'- -UGGACCacuUGCUGUAGUGGaguugc -5'
8898 5' -51.3 NC_002484.1 + 24845 0.67 0.885969
Target:  5'- gACCUGc-GGGCGGC-UCGCCgcUCGGCGg -3'
miRNA:   3'- -UGGACcaCUUGCUGuAGUGG--AGUUGC- -5'
8898 5' -51.3 NC_002484.1 + 35610 0.67 0.853515
Target:  5'- cGCCUgauuGGUGAGCGG-AUCACCggAGCa -3'
miRNA:   3'- -UGGA----CCACUUGCUgUAGUGGagUUGc -5'
8898 5' -51.3 NC_002484.1 + 26723 0.68 0.826598
Target:  5'- cACCUGGUGGuCGAguuUCACCgacCAGCc -3'
miRNA:   3'- -UGGACCACUuGCUgu-AGUGGa--GUUGc -5'
8898 5' -51.3 NC_002484.1 + 38240 0.69 0.792779
Target:  5'- gACCUGGUGAugccggccggcgcCGAUAUCGCCgcugugcUCGGCc -3'
miRNA:   3'- -UGGACCACUu------------GCUGUAGUGG-------AGUUGc -5'
8898 5' -51.3 NC_002484.1 + 51383 0.7 0.735488
Target:  5'- cGCCUGGcaGAACaGGCAUaacCAgCUCAACGg -3'
miRNA:   3'- -UGGACCa-CUUG-CUGUA---GUgGAGUUGC- -5'
8898 5' -51.3 NC_002484.1 + 33014 0.7 0.729002
Target:  5'- uGCgaGGUGAGCGAagacgagcugaugacCGUCGCC-CAGCa -3'
miRNA:   3'- -UGgaCCACUUGCU---------------GUAGUGGaGUUGc -5'
8898 5' -51.3 NC_002484.1 + 40641 0.71 0.658071
Target:  5'- uGCCUGGUGGAacuuugaGGCcgguuucaaucuGgcgCGCCUCAACGg -3'
miRNA:   3'- -UGGACCACUUg------CUG------------Ua--GUGGAGUUGC- -5'
8898 5' -51.3 NC_002484.1 + 35372 0.76 0.402139
Target:  5'- -gCUGGUGAGCGugAUCAUCgcaGGCGa -3'
miRNA:   3'- ugGACCACUUGCugUAGUGGag-UUGC- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.