Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 890 | 3' | -52.2 | NC_000899.1 | + | 10365 | 0.67 | 0.778635 |
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Target: 5'- aCCgGUAggcgCGGAACCGCCGGgcaacgaagccGGGCc -3' miRNA: 3'- gGGaCAUa---GUUUUGGCGGCCac---------CUCG- -5' |
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| 890 | 3' | -52.2 | NC_000899.1 | + | 153 | 0.67 | 0.770378 |
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Target: 5'- aCUUUGUAUCAcgGAAauaCuuUGGUGGAGCa -3' miRNA: 3'- -GGGACAUAGU--UUUg--GcgGCCACCUCG- -5' |
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| 890 | 3' | -52.2 | NC_000899.1 | + | 43465 | 0.68 | 0.759923 |
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Target: 5'- cUCCUGUccgUAGAGCCagcuCCGGgucccggGGAGCg -3' miRNA: 3'- -GGGACAua-GUUUUGGc---GGCCa------CCUCG- -5' |
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| 890 | 3' | -52.2 | NC_000899.1 | + | 28088 | 0.68 | 0.746127 |
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Target: 5'- cCCCUGgcagcuccagGUCGAGccucgccgacgcucGCCGCCGaaGUGGcuAGCg -3' miRNA: 3'- -GGGACa---------UAGUUU--------------UGGCGGC--CACC--UCG- -5' |
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| 890 | 3' | -52.2 | NC_000899.1 | + | 9788 | 0.68 | 0.716847 |
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Target: 5'- gUCUUGU-UCGuAACCGCCGaUGGAGa -3' miRNA: 3'- -GGGACAuAGUuUUGGCGGCcACCUCg -5' |
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| 890 | 3' | -52.2 | NC_000899.1 | + | 1358 | 1.13 | 0.000894 |
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Target: 5'- cCCCUGUAUCAAAACCGCCGGUGGAGCc -3' miRNA: 3'- -GGGACAUAGUUUUGGCGGCCACCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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