Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8905 | 3' | -56.9 | NC_002484.1 | + | 41693 | 0.66 | 0.652984 |
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Target: 5'- aUAUCGCUGGCCG-AGcugcucGCCG-CCGcGCCc -3' miRNA: 3'- -GUGGCGACUGGCaUC------UGGCaGGC-UGG- -5' |
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| 8905 | 3' | -56.9 | NC_002484.1 | + | 55045 | 0.67 | 0.631578 |
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Target: 5'- uUACCGCUGGCaCG-AGACCaucguugCUGACg -3' miRNA: 3'- -GUGGCGACUG-GCaUCUGGca-----GGCUGg -5' |
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| 8905 | 3' | -56.9 | NC_002484.1 | + | 34115 | 0.67 | 0.631578 |
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Target: 5'- uCACCGCccUGGCgGUGuGACgGUcaCCGGCUg -3' miRNA: 3'- -GUGGCG--ACUGgCAU-CUGgCA--GGCUGG- -5' |
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| 8905 | 3' | -56.9 | NC_002484.1 | + | 39238 | 0.67 | 0.587776 |
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Target: 5'- aACUGCUGGaaagCGUgaagcaggcGGACCagaucauGUCCGACCa -3' miRNA: 3'- gUGGCGACUg---GCA---------UCUGG-------CAGGCUGG- -5' |
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| 8905 | 3' | -56.9 | NC_002484.1 | + | 5366 | 0.68 | 0.57822 |
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Target: 5'- aUACCGCUGGCCG-GGAac--UCGGCCa -3' miRNA: 3'- -GUGGCGACUGGCaUCUggcaGGCUGG- -5' |
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| 8905 | 3' | -56.9 | NC_002484.1 | + | 26523 | 0.69 | 0.475465 |
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Target: 5'- aGCCGCgGGCC--AGGCCGgcaccUCGACCg -3' miRNA: 3'- gUGGCGaCUGGcaUCUGGCa----GGCUGG- -5' |
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| 8905 | 3' | -56.9 | NC_002484.1 | + | 41261 | 0.75 | 0.207798 |
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Target: 5'- gACUgGCUGAUCGUugAGGCCGuucggUCCGACCg -3' miRNA: 3'- gUGG-CGACUGGCA--UCUGGC-----AGGCUGG- -5' |
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| 8905 | 3' | -56.9 | NC_002484.1 | + | 14155 | 0.76 | 0.192215 |
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Target: 5'- uGCCGCUGACCagcuuUGGACCGUCauaGAUg -3' miRNA: 3'- gUGGCGACUGGc----AUCUGGCAGg--CUGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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