Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8905 | 5' | -54.9 | NC_002484.1 | + | 29304 | 0.66 | 0.750226 |
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Target: 5'- gCGGCGCGGGcGGCGGgGUau-CAGUu -3' miRNA: 3'- gGUCGCGCCC-CUGUUgUAgauGUCGc -5' |
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| 8905 | 5' | -54.9 | NC_002484.1 | + | 8721 | 0.67 | 0.675644 |
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Target: 5'- gCAGCGCccaucGGGG-UAGCGUCaaGCAGCu -3' miRNA: 3'- gGUCGCG-----CCCCuGUUGUAGa-UGUCGc -5' |
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| 8905 | 5' | -54.9 | NC_002484.1 | + | 19070 | 0.68 | 0.631879 |
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Target: 5'- gCCAGCGCuGGuGGcCAGgGUgucUUGCAGCGu -3' miRNA: 3'- -GGUCGCG-CC-CCuGUUgUA---GAUGUCGC- -5' |
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| 8905 | 5' | -54.9 | NC_002484.1 | + | 10930 | 0.7 | 0.522652 |
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Target: 5'- uCCAGCGCGccgcgaccGGGAUcggccucgccgguGAUAUCUGcCGGCGu -3' miRNA: 3'- -GGUCGCGC--------CCCUG-------------UUGUAGAU-GUCGC- -5' |
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| 8905 | 5' | -54.9 | NC_002484.1 | + | 40586 | 0.7 | 0.492501 |
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Target: 5'- gUCAGCGaGGGGcuguuGCGGCGUCUG-AGCGa -3' miRNA: 3'- -GGUCGCgCCCC-----UGUUGUAGAUgUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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