miRNA: 3'- caAUGGcGAG-ACGGCUA-GCUUCUGGc -5'
miRNA: 3'- caaUGGCgAGACGGCuaGCUUCUgGc -5'
miRNA: 3'- caaUGGCGAGACGGCU-AGCUucUGgc -5'
miRNA: 3'- caAUGG--CGAGACGGCUA-GCuUCugGC- -5'
miRNA: 3'- caaUGGCGA---------GACGGCuaGCuUCUGGc -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8916 | 5' | -53.4 | NC_002484.1 | + | 11153 | 0.66 | 0.811741 |
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Target: 5'- -cUACC-CUCaUGCCGAUaUGGAGACUc -3' miRNA: 3'- caAUGGcGAG-ACGGCUA-GCUUCUGGc -5' |
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| 8916 | 5' | -53.4 | NC_002484.1 | + | 43227 | 0.66 | 0.802159 |
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Target: 5'- --gGCCGaUCUGCUGcgCGAGGAgCu -3' miRNA: 3'- caaUGGCgAGACGGCuaGCUUCUgGc -5' |
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| 8916 | 5' | -53.4 | NC_002484.1 | + | 17633 | 0.67 | 0.782465 |
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Target: 5'- --cACCGUUCUGCCGAgUCGGuaGCa- -3' miRNA: 3'- caaUGGCGAGACGGCU-AGCUucUGgc -5' |
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| 8916 | 5' | -53.4 | NC_002484.1 | + | 25422 | 0.67 | 0.751761 |
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Target: 5'- -cUAUCugGCUCUGCCGGUgCG-AGugCGg -3' miRNA: 3'- caAUGG--CGAGACGGCUA-GCuUCugGC- -5' |
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| 8916 | 5' | -53.4 | NC_002484.1 | + | 38211 | 0.69 | 0.676332 |
|
Target: 5'- --cGCCGCUgugcucggcCUGCCGcgCG-AGACCc -3' miRNA: 3'- caaUGGCGA---------GACGGCuaGCuUCUGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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