Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8920 | 3' | -53.9 | NC_002484.1 | + | 18917 | 0.73 | 0.373388 |
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Target: 5'- cGGACGGUCuacggUCAGCGGUgAGCC-GUCGa -3' miRNA: 3'- -CUUGCCGG-----AGUUGCUAgUCGGuCAGC- -5' |
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| 8920 | 3' | -53.9 | NC_002484.1 | + | 50767 | 0.72 | 0.419354 |
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Target: 5'- uGAACGGUUUC-GCGAUCuGCCucGGUCGc -3' miRNA: 3'- -CUUGCCGGAGuUGCUAGuCGG--UCAGC- -5' |
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| 8920 | 3' | -53.9 | NC_002484.1 | + | 31939 | 0.72 | 0.458499 |
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Target: 5'- cAACGGCCU-GACGGUCAGCgAGg-- -3' miRNA: 3'- cUUGCCGGAgUUGCUAGUCGgUCagc -5' |
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| 8920 | 3' | -53.9 | NC_002484.1 | + | 14572 | 0.71 | 0.489099 |
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Target: 5'- cGAUGGCCUCGGCaauauacugcuGAgcaaAGCCAGUCa -3' miRNA: 3'- cUUGCCGGAGUUG-----------CUag--UCGGUCAGc -5' |
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| 8920 | 3' | -53.9 | NC_002484.1 | + | 6334 | 0.71 | 0.51002 |
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Target: 5'- cGGACGGCCacUCcGCGAUCuuGCCGG-CGc -3' miRNA: 3'- -CUUGCCGG--AGuUGCUAGu-CGGUCaGC- -5' |
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| 8920 | 3' | -53.9 | NC_002484.1 | + | 5021 | 0.69 | 0.641193 |
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Target: 5'- gGAGCGGCauugUUGACGA--AGCCGGUCu -3' miRNA: 3'- -CUUGCCGg---AGUUGCUagUCGGUCAGc -5' |
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| 8920 | 3' | -53.9 | NC_002484.1 | + | 40609 | 0.67 | 0.728847 |
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Target: 5'- uGGCGcGCCUCAACGGcaagaCGGUCAG-CGa -3' miRNA: 3'- cUUGC-CGGAGUUGCUa----GUCGGUCaGC- -5' |
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| 8920 | 3' | -53.9 | NC_002484.1 | + | 35430 | 0.66 | 0.760411 |
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Target: 5'- cGGAUGGCCuagUCGGCGAcaaCGGCCuGaUCGa -3' miRNA: 3'- -CUUGCCGG---AGUUGCUa--GUCGGuC-AGC- -5' |
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| 8920 | 3' | -53.9 | NC_002484.1 | + | 16970 | 0.66 | 0.79077 |
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Target: 5'- gGAGcCGGUCUgCAACGcAUUGGCCGG-CGa -3' miRNA: 3'- -CUU-GCCGGA-GUUGC-UAGUCGGUCaGC- -5' |
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| 8920 | 3' | -53.9 | NC_002484.1 | + | 36715 | 0.66 | 0.810187 |
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Target: 5'- cAACGGCaggcCGACGAgcaGGCCAGgaUCGa -3' miRNA: 3'- cUUGCCGga--GUUGCUag-UCGGUC--AGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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