miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
8922 5' -55.2 NC_002484.1 + 4365 0.66 0.719287
Target:  5'- uGAGUucauuGCGcugGCUUCGCGGCAUuccgcugCGAGCc -3'
miRNA:   3'- gCUCGu----CGC---UGAGGUGUCGUA-------GCUCG- -5'
8922 5' -55.2 NC_002484.1 + 6955 0.66 0.741486
Target:  5'- -uGGCAGUGGuCUCCGCcugcguAGCGUcCGcAGCg -3'
miRNA:   3'- gcUCGUCGCU-GAGGUG------UCGUA-GC-UCG- -5'
8922 5' -55.2 NC_002484.1 + 48807 0.67 0.644224
Target:  5'- gCGGGCaauggAGCGGaUUCGCGGCAUCGucaugaccGGCa -3'
miRNA:   3'- -GCUCG-----UCGCUgAGGUGUCGUAGC--------UCG- -5'
8922 5' -55.2 NC_002484.1 + 23853 0.69 0.513033
Target:  5'- aGAGUAGCGAaucugcuugaUCUuCAGCAugacaguUCGAGCa -3'
miRNA:   3'- gCUCGUCGCUg---------AGGuGUCGU-------AGCUCG- -5'
8922 5' -55.2 NC_002484.1 + 33367 0.69 0.556683
Target:  5'- uCGGGCAaGCG-CUCgGCGaguGCAUCGaAGCu -3'
miRNA:   3'- -GCUCGU-CGCuGAGgUGU---CGUAGC-UCG- -5'
8922 5' -55.2 NC_002484.1 + 18356 0.7 0.462828
Target:  5'- aGAGCgcuGGCGAgUUCAgCAGUgaccaGUCGAGCg -3'
miRNA:   3'- gCUCG---UCGCUgAGGU-GUCG-----UAGCUCG- -5'
8922 5' -55.2 NC_002484.1 + 16350 0.71 0.433392
Target:  5'- aCGGcCAGCGACUUCGCGgaucGCAUcgcCGAGCu -3'
miRNA:   3'- -GCUcGUCGCUGAGGUGU----CGUA---GCUCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.