miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
8924 3' -58.4 NC_002484.1 + 14179 0.66 0.505795
Target:  5'- cGgUGaaaUCCCAGaGCGa-GAUACUGCCg -3'
miRNA:   3'- aCgACg--AGGGUC-CGUccCUAUGACGG- -5'
8924 3' -58.4 NC_002484.1 + 26320 0.66 0.515131
Target:  5'- cGCUGaCUCagCAGGCGuuGGGcuccggcAUACUGCUc -3'
miRNA:   3'- aCGAC-GAGg-GUCCGU--CCC-------UAUGACGG- -5'
8924 3' -58.4 NC_002484.1 + 37303 0.67 0.426447
Target:  5'- cUGCUGgaCUCCuggCAGGCAGaGGUAC-GCCa -3'
miRNA:   3'- -ACGAC--GAGG---GUCCGUCcCUAUGaCGG- -5'
8924 3' -58.4 NC_002484.1 + 51780 0.68 0.407783
Target:  5'- cGCUGC-CCCAGGCGcuGcGGAacgaacugGgUGCCg -3'
miRNA:   3'- aCGACGaGGGUCCGU--C-CCUa-------UgACGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.