miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
8926 3' -47.4 NC_002484.1 + 40458 0.68 0.928476
Target:  5'- cAGGGGAAGGAGcaugagugagcugaaGUCuUGCCCCCUGu-- -3'
miRNA:   3'- -UUUCUUUCUUC---------------UAG-AUGGGGGACuuu -5'
8926 3' -47.4 NC_002484.1 + 47424 0.67 0.965855
Target:  5'- -cGGAgcGu-GAUCaGCCCCCUGggGg -3'
miRNA:   3'- uuUCUuuCuuCUAGaUGGGGGACuuU- -5'
8926 3' -47.4 NC_002484.1 + 49205 0.66 0.978639
Target:  5'- aGAGGAAcGAaucgGGAUUUGCuUCCCUGGAAg -3'
miRNA:   3'- -UUUCUUuCU----UCUAGAUG-GGGGACUUU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.