Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8930 | 5' | -47.9 | NC_002484.1 | + | 24991 | 0.74 | 0.740451 |
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Target: 5'- uCCGagaACCAGCGAcgCCugaccgGAGUCGAGAu -3' miRNA: 3'- -GGUga-UGGUUGCUuaGG------UUCAGCUCU- -5' |
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| 8930 | 5' | -47.9 | NC_002484.1 | + | 16417 | 0.7 | 0.889026 |
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Target: 5'- uCCACUgcucacgcggACCGGCGAAauacuUCCGgggcggcaggauguAGUCGGGAu -3' miRNA: 3'- -GGUGA----------UGGUUGCUU-----AGGU--------------UCAGCUCU- -5' |
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| 8930 | 5' | -47.9 | NC_002484.1 | + | 16666 | 0.7 | 0.918992 |
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Target: 5'- --gUUGCCGACGggUUCGGGgUGAGAu -3' miRNA: 3'- gguGAUGGUUGCuuAGGUUCaGCUCU- -5' |
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| 8930 | 5' | -47.9 | NC_002484.1 | + | 8978 | 0.69 | 0.931065 |
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Target: 5'- gCCACUcGCCAACGuGGUCCAgcAGUuucuccggcucCGGGAu -3' miRNA: 3'- -GGUGA-UGGUUGC-UUAGGU--UCA-----------GCUCU- -5' |
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| 8930 | 5' | -47.9 | NC_002484.1 | + | 54775 | 0.68 | 0.956056 |
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Target: 5'- cCCACUACCAACGcacagCAGGUaugCGAGc -3' miRNA: 3'- -GGUGAUGGUUGCuuag-GUUCA---GCUCu -5' |
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| 8930 | 5' | -47.9 | NC_002484.1 | + | 36717 | 0.68 | 0.967632 |
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Target: 5'- gCCAacggcagGCCGACGAGcaggCCAGGaUCGAGc -3' miRNA: 3'- -GGUga-----UGGUUGCUUa---GGUUC-AGCUCu -5' |
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| 8930 | 5' | -47.9 | NC_002484.1 | + | 5486 | 0.67 | 0.974032 |
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Target: 5'- uCCACcACCGGCaGAcgCCAgagGGUCG-GAg -3' miRNA: 3'- -GGUGaUGGUUG-CUuaGGU---UCAGCuCU- -5' |
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| 8930 | 5' | -47.9 | NC_002484.1 | + | 27523 | 0.67 | 0.976862 |
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Target: 5'- gCACUAaagCGACGAauggcAUUCAGGUcCGAGAc -3' miRNA: 3'- gGUGAUg--GUUGCU-----UAGGUUCA-GCUCU- -5' |
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| 8930 | 5' | -47.9 | NC_002484.1 | + | 32117 | 0.66 | 0.987726 |
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Target: 5'- aCAagGCCAAUGAgcugaaGUCCgAAGUCGAGc -3' miRNA: 3'- gGUgaUGGUUGCU------UAGG-UUCAGCUCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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