Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8932 | 5' | -57.7 | NC_002484.1 | + | 15947 | 0.7 | 0.316364 |
Target: 5'- cGGCCgaucgGAUGCC-GCUCCAGCGGu -3' miRNA: 3'- uCCGGaca--UUGCGGcCGAGGUCGUUc -5' |
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8932 | 5' | -57.7 | NC_002484.1 | + | 27908 | 0.7 | 0.32417 |
Target: 5'- cAGGCUg--GAUGCCGGCgcauccCCGGCAAc -3' miRNA: 3'- -UCCGGacaUUGCGGCCGa-----GGUCGUUc -5' |
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8932 | 5' | -57.7 | NC_002484.1 | + | 39352 | 0.68 | 0.457834 |
Target: 5'- uGGCC-GUGGCGUCguugaugucaaGGCUCaaCAGCGAGa -3' miRNA: 3'- uCCGGaCAUUGCGG-----------CCGAG--GUCGUUC- -5' |
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8932 | 5' | -57.7 | NC_002484.1 | + | 17540 | 0.67 | 0.48795 |
Target: 5'- cGGCCauuguUGUGACGCCGGCguaGGaCGAc -3' miRNA: 3'- uCCGG-----ACAUUGCGGCCGaggUC-GUUc -5' |
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8932 | 5' | -57.7 | NC_002484.1 | + | 27350 | 0.66 | 0.54007 |
Target: 5'- uGGCUgGUcAGCGCCGGCguuccgCUGGCGGa -3' miRNA: 3'- uCCGGaCA-UUGCGGCCGa-----GGUCGUUc -5' |
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8932 | 5' | -57.7 | NC_002484.1 | + | 42470 | 0.66 | 0.550731 |
Target: 5'- --uCCUGUauGACGCCGGCaccgccgCCAGgAGGa -3' miRNA: 3'- uccGGACA--UUGCGGCCGa------GGUCgUUC- -5' |
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8932 | 5' | -57.7 | NC_002484.1 | + | 54237 | 0.66 | 0.572237 |
Target: 5'- uGGGCCuUGUAGuauggUGCCGGC---AGCAGGa -3' miRNA: 3'- -UCCGG-ACAUU-----GCGGCCGaggUCGUUC- -5' |
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8932 | 5' | -57.7 | NC_002484.1 | + | 52400 | 0.66 | 0.583066 |
Target: 5'- uGGCC---GAUGCCGGa--CAGCAGGg -3' miRNA: 3'- uCCGGacaUUGCGGCCgagGUCGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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