miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
8934 5' -60.4 NC_002484.1 + 17765 0.7 0.280141
Target:  5'- cGCGGGCCUUCCagauugcgUCCAGCCgauCGAg -3'
miRNA:   3'- uCGCCUGGAAGGgcaa----GGGUCGG---GCU- -5'
8934 5' -60.4 NC_002484.1 + 26563 0.69 0.321185
Target:  5'- cGCGGACCUuggucuggagaucgUCgCCcUUCCgCAGCCCa- -3'
miRNA:   3'- uCGCCUGGA--------------AG-GGcAAGG-GUCGGGcu -5'
8934 5' -60.4 NC_002484.1 + 8287 0.68 0.331155
Target:  5'- gAGUGGAUCgauggCCCaUUCCUuGCCCGGc -3'
miRNA:   3'- -UCGCCUGGaa---GGGcAAGGGuCGGGCU- -5'
8934 5' -60.4 NC_002484.1 + 3087 0.66 0.461951
Target:  5'- cGgGGAUCUUCCggaGUUCaUCGcGCCCGAu -3'
miRNA:   3'- uCgCCUGGAAGGg--CAAG-GGU-CGGGCU- -5'
8934 5' -60.4 NC_002484.1 + 17947 0.66 0.480404
Target:  5'- cAGUGGACCaUCCaUGUUCugcggcgaguacaUguGCCCGAa -3'
miRNA:   3'- -UCGCCUGGaAGG-GCAAG-------------GguCGGGCU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.