Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8936 | 3' | -52.9 | NC_002484.1 | + | 3428 | 0.69 | 0.581251 |
Target: 5'- gUGCUGuaccaGGCAGCGC--GACCAgcgcuAUCAGc -3' miRNA: 3'- aACGAC-----UCGUCGCGaaCUGGU-----UAGUC- -5' |
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8936 | 3' | -52.9 | NC_002484.1 | + | 4280 | 0.68 | 0.672994 |
Target: 5'- gUUGCUGGG-AGCGCUggaGAUCucGUCGGa -3' miRNA: 3'- -AACGACUCgUCGCGAa--CUGGu-UAGUC- -5' |
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8936 | 3' | -52.9 | NC_002484.1 | + | 18329 | 0.67 | 0.740331 |
Target: 5'- -aGUcGAGCGGCucGCUgGACuCGAUCAGg -3' miRNA: 3'- aaCGaCUCGUCG--CGAaCUG-GUUAGUC- -5' |
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8936 | 3' | -52.9 | NC_002484.1 | + | 38127 | 0.66 | 0.761962 |
Target: 5'- gUGCcugGGGguGCGUcgUGACUGAUCAu -3' miRNA: 3'- aACGa--CUCguCGCGa-ACUGGUUAGUc -5' |
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8936 | 3' | -52.9 | NC_002484.1 | + | 25327 | 0.66 | 0.761962 |
Target: 5'- -aGCgaaUGGGCuucAGCGCUUGGCuCAAaCAGg -3' miRNA: 3'- aaCG---ACUCG---UCGCGAACUG-GUUaGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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