miRNA: 3'- -GCgACCGCCGCUGuCACGaU----------AGCAGC- -5'
miRNA: 3'- -GCG--ACCG---CCGCUGUCACGAuaG-CAGc -5'
miRNA: 3'- -GCGa----CCGCCGCUGUCa-CGa-----UAGCaGC- -5'
miRNA: 3'- gCgaCCGCCGCUGUCaCGAUa----------GCAGc -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8955 | 3' | -56.8 | NC_002512.2 | + | 20299 | 0.67 | 0.907244 |
|
Target: 5'- aCG-UGGCGGUGACcGUGCcGgacgaggagcUCGUCGa -3' miRNA: 3'- -GCgACCGCCGCUGuCACGaU----------AGCAGC- -5' |
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| 8955 | 3' | -56.8 | NC_002512.2 | + | 15328 | 0.66 | 0.93876 |
|
Target: 5'- uCGCccUGGUcgcGGUGACAGUGCUcgCaGUUa -3' miRNA: 3'- -GCG--ACCG---CCGCUGUCACGAuaG-CAGc -5' |
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| 8955 | 3' | -56.8 | NC_002512.2 | + | 12390 | 0.68 | 0.860647 |
|
Target: 5'- uCGCcuucgGGCGGCGGCGGcgGCgccgggAUCG-CGa -3' miRNA: 3'- -GCGa----CCGCCGCUGUCa-CGa-----UAGCaGC- -5' |
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| 8955 | 3' | -56.8 | NC_002512.2 | + | 6407 | 0.69 | 0.845539 |
|
Target: 5'- gGgaGGCGGCGGCGGcGCUGgggaaggcggcCGUCu -3' miRNA: 3'- gCgaCCGCCGCUGUCaCGAUa----------GCAGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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