miRNA: 3'- -CGaGCGuGGGCGAGUACCGCC-Ca---GUC- -5'
miRNA: 3'- -CGAGCGUGGGC-GAGuacCGCCCAGUC- -5'
miRNA: 3'- -CGAGCGUGGGCGAGUACCGCCCAGUC- -5'
Results 41 - 43 of 43 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8956 | 5' | -59.5 | NC_002512.2 | + | 20868 | 0.74 | 0.414412 |
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Target: 5'- gGC-CGC-CCCGCUCAUGGCGGcGacgaCGGg -3' miRNA: 3'- -CGaGCGuGGGCGAGUACCGCC-Ca---GUC- -5' |
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| 8956 | 5' | -59.5 | NC_002512.2 | + | 45279 | 0.77 | 0.270511 |
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Target: 5'- cGCUCGCGCUCGuCUC---GCGGGUCGGa -3' miRNA: 3'- -CGAGCGUGGGC-GAGuacCGCCCAGUC- -5' |
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| 8956 | 5' | -59.5 | NC_002512.2 | + | 213147 | 1.09 | 0.002076 |
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Target: 5'- cGCUCGCACCCGCUCAUGGCGGGUCAGc -3' miRNA: 3'- -CGAGCGUGGGCGAGUACCGCCCAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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