Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
896 | 3' | -51.1 | NC_000899.1 | + | 14387 | 0.66 | 0.852798 |
Target: 5'- aUCCAGc---GGCGGcGAGGCGGAGgAg -3' miRNA: 3'- aAGGUCucauUCGCC-UUUCGCCUUgU- -5' |
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896 | 3' | -51.1 | NC_000899.1 | + | 27579 | 0.66 | 0.834348 |
Target: 5'- aUCCcacaagAGAGgugcgcuuuGGCGGAAggcuAGCGGAAUAa -3' miRNA: 3'- aAGG------UCUCau-------UCGCCUU----UCGCCUUGU- -5' |
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896 | 3' | -51.1 | NC_000899.1 | + | 7377 | 0.66 | 0.824757 |
Target: 5'- -cCCAuGAGcgGAGCGGAGGcCGGGGCc -3' miRNA: 3'- aaGGU-CUCa-UUCGCCUUUcGCCUUGu -5' |
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896 | 3' | -51.1 | NC_000899.1 | + | 35651 | 0.67 | 0.794659 |
Target: 5'- gUCCggaAGAGUAuGCGGAGAaaauguuuGgGGAGCGa -3' miRNA: 3'- aAGG---UCUCAUuCGCCUUU--------CgCCUUGU- -5' |
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896 | 3' | -51.1 | NC_000899.1 | + | 4393 | 0.67 | 0.784226 |
Target: 5'- -aCgGGGGUGAGCucuuGGGGAGCcgugaGGAACAg -3' miRNA: 3'- aaGgUCUCAUUCG----CCUUUCG-----CCUUGU- -5' |
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896 | 3' | -51.1 | NC_000899.1 | + | 18541 | 0.67 | 0.784226 |
Target: 5'- ---gGGAGUcuucGGCGGGAAGCGGuuGACGc -3' miRNA: 3'- aaggUCUCAu---UCGCCUUUCGCC--UUGU- -5' |
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896 | 3' | -51.1 | NC_000899.1 | + | 11173 | 0.68 | 0.706955 |
Target: 5'- uUUCUAGGGgaGGCGGGGAguacGCGGcACGg -3' miRNA: 3'- -AAGGUCUCauUCGCCUUU----CGCCuUGU- -5' |
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896 | 3' | -51.1 | NC_000899.1 | + | 38586 | 0.69 | 0.695476 |
Target: 5'- gUCCuucGGUGGGCGGGgcucuggauucgGGGUGGAGCGu -3' miRNA: 3'- aAGGuc-UCAUUCGCCU------------UUCGCCUUGU- -5' |
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896 | 3' | -51.1 | NC_000899.1 | + | 11993 | 0.7 | 0.590585 |
Target: 5'- cUUCgGGAG-GGGCcGggGGCGGAACGa -3' miRNA: 3'- -AAGgUCUCaUUCGcCuuUCGCCUUGU- -5' |
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896 | 3' | -51.1 | NC_000899.1 | + | 37290 | 0.7 | 0.590585 |
Target: 5'- gUCCAG-GUAAaugauuuacCGGGAGGCGGAGCu -3' miRNA: 3'- aAGGUCuCAUUc--------GCCUUUCGCCUUGu -5' |
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896 | 3' | -51.1 | NC_000899.1 | + | 5293 | 1.06 | 0.002562 |
Target: 5'- cUUCCAGAGUAAGCGGAAAGCGGAACAu -3' miRNA: 3'- -AAGGUCUCAUUCGCCUUUCGCCUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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