Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 896 | 5' | -63.6 | NC_000899.1 | + | 11306 | 0.66 | 0.28996 |
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Target: 5'- gCUCggcacgCgCCUCCUCGCaugcgaugaGCCUCuCGUCGu -3' miRNA: 3'- gGAGa-----G-GGAGGAGCGg--------CGGGG-GCAGC- -5' |
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| 896 | 5' | -63.6 | NC_000899.1 | + | 43860 | 0.67 | 0.269889 |
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Target: 5'- -aUCUCUCUUC-CGCCacGCCCUCGUUa -3' miRNA: 3'- ggAGAGGGAGGaGCGG--CGGGGGCAGc -5' |
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| 896 | 5' | -63.6 | NC_000899.1 | + | 43975 | 0.67 | 0.263452 |
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Target: 5'- aCUCUUCCgaauccaCUUCGCCGCCUUCGa-- -3' miRNA: 3'- gGAGAGGGa------GGAGCGGCGGGGGCagc -5' |
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| 896 | 5' | -63.6 | NC_000899.1 | + | 3152 | 0.67 | 0.250952 |
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Target: 5'- aCCUCUaCUCaUCUCcaCCGCCCCCGaCGa -3' miRNA: 3'- -GGAGA-GGGaGGAGc-GGCGGGGGCaGC- -5' |
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| 896 | 5' | -63.6 | NC_000899.1 | + | 16327 | 0.68 | 0.211554 |
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Target: 5'- uCCUCUUCCUCCuUCgcgacgucgaugccgGCCGCUCCUccuccuucgccuguGUCGg -3' miRNA: 3'- -GGAGAGGGAGG-AG---------------CGGCGGGGG--------------CAGC- -5' |
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| 896 | 5' | -63.6 | NC_000899.1 | + | 14457 | 0.69 | 0.205789 |
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Target: 5'- uCCUCcUCCgCCUCGCCGCCgCUGg-- -3' miRNA: 3'- -GGAGaGGGaGGAGCGGCGGgGGCagc -5' |
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| 896 | 5' | -63.6 | NC_000899.1 | + | 25229 | 0.69 | 0.185958 |
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Target: 5'- aCCUgUCgaaUCUCCUCGUCGUCUUCGUCc -3' miRNA: 3'- -GGAgAG---GGAGGAGCGGCGGGGGCAGc -5' |
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| 896 | 5' | -63.6 | NC_000899.1 | + | 30387 | 0.7 | 0.151306 |
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Target: 5'- gCCgCUUCCUCCUCcuCCGCCCCCc--- -3' miRNA: 3'- -GGaGAGGGAGGAGc-GGCGGGGGcagc -5' |
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| 896 | 5' | -63.6 | NC_000899.1 | + | 8018 | 0.75 | 0.067778 |
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Target: 5'- cCCUCUCCUUCaugCGCCGauaCCCCGacUCGg -3' miRNA: 3'- -GGAGAGGGAGga-GCGGCg--GGGGC--AGC- -5' |
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| 896 | 5' | -63.6 | NC_000899.1 | + | 5259 | 1.09 | 0.000169 |
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Target: 5'- cCCUCUCCCUCCUCGCCGCCCCCGUCGc -3' miRNA: 3'- -GGAGAGGGAGGAGCGGCGGGGGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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