miRNA: 3'- -CUCUaca---UGCUGgaCGCCGGCCUGCu -5'
miRNA: 3'- -CUCUaCA--UGCuGGaCgCCGGCCUGCU- -5'
miRNA: 3'- -CUCUacauGCUGGaCGCCGG--CCUGCU- -5'
miRNA: 3'- cUCUACAU-GCuGGaCGC-CGGC-CUGCU- -5'
miRNA: 3'- cucuacaUGCUGGACG-CCGGC--------CUGCU- -5'
Results 81 - 85 of 85 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8963 | 5' | -56.9 | NC_002512.2 | + | 111218 | 0.67 | 0.902264 |
|
Target: 5'- cGGGAcagacgAUGACggGCGGCCGGGCGc -3' miRNA: 3'- -CUCUaca---UGCUGgaCGCCGGCCUGCu -5' |
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| 8963 | 5' | -56.9 | NC_002512.2 | + | 191664 | 0.67 | 0.914012 |
|
Target: 5'- gGAGAcGUccGCGuCCcGgGGCUGGGCGAc -3' miRNA: 3'- -CUCUaCA--UGCuGGaCgCCGGCCUGCU- -5' |
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| 8963 | 5' | -56.9 | NC_002512.2 | + | 121152 | 0.67 | 0.919557 |
|
Target: 5'- cGAGAcgc-CGGCC-GCGGCCcaGGGCGAg -3' miRNA: 3'- -CUCUacauGCUGGaCGCCGG--CCUGCU- -5' |
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| 8963 | 5' | -56.9 | NC_002512.2 | + | 225713 | 0.67 | 0.919557 |
|
Target: 5'- aGGAUGUGuCG-CCcGCGaGCCGcGGCGAc -3' miRNA: 3'- cUCUACAU-GCuGGaCGC-CGGC-CUGCU- -5' |
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| 8963 | 5' | -56.9 | NC_002512.2 | + | 146938 | 0.66 | 0.948177 |
|
Target: 5'- ------cGCGACCUGCuGGCCGugaagcgcGACGAc -3' miRNA: 3'- cucuacaUGCUGGACG-CCGGC--------CUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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