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miRNA: 3'- uaCUUGCCGa--CCGUGUGGCGGCGcaa -5'
miRNA: 3'- uaCUUGCCGaCCGUGUGGCGG-CGCaa -5'
miRNA: 3'- uacUUGCCGa-----CCGUGUGGCGGCGCaa -5'
miRNA: 3'- uaCUUGCCGACCGUG-UGGCGGCGCaa -5'
miRNA: 3'- -UACUUGCCGACCGUGUGGCGGCGCAA- -5'
Results 81 - 85 of 85 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 8966 | 5' | -59.6 | NC_002512.2 | + | 148833 | 0.74 | 0.357379 |
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Target: 5'- -gGGGCGGUccgGGCGCACCGCUGCc-- -3' miRNA: 3'- uaCUUGCCGa--CCGUGUGGCGGCGcaa -5' |
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| 8966 | 5' | -59.6 | NC_002512.2 | + | 91914 | 0.74 | 0.372939 |
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Target: 5'- -gGGGCGGC-GGCGCGCCGUCgGCGg- -3' miRNA: 3'- uaCUUGCCGaCCGUGUGGCGG-CGCaa -5' |
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| 8966 | 5' | -59.6 | NC_002512.2 | + | 150536 | 0.75 | 0.320507 |
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Target: 5'- --cGGCGGCcgcgagGGUACGCCGCCGCGa- -3' miRNA: 3'- uacUUGCCGa-----CCGUGUGGCGGCGCaa -5' |
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| 8966 | 5' | -59.6 | NC_002512.2 | + | 69083 | 0.78 | 0.206623 |
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Target: 5'- -gGGGCGGCUGGUACuGCUGCCGUGg- -3' miRNA: 3'- uaCUUGCCGACCGUG-UGGCGGCGCaa -5' |
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| 8966 | 5' | -59.6 | NC_002512.2 | + | 206930 | 1.04 | 0.003828 |
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Target: 5'- gAUGAACGGCUGGCACACCGCCGCGUUg -3' miRNA: 3'- -UACUUGCCGACCGUGUGGCGGCGCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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