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miRNA: 3'- uaCuuGCCGa------CCGUGUGGCGGCGcaa -5'
miRNA: 3'- -UACUUGCCGAC--CGUGUGG--------------CGGCGcaa -5'
miRNA: 3'- uaCUUGCCGaCCG---UGuGGCGGCGcaa -5'
miRNA: 3'- uacUUGCCGaCCG----UGUGGC-GGCGCaa -5'
miRNA: 3'- uaCUUGCCgaCCGuGUGGCGGCgcaa -5'
Results 81 - 85 of 85 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 8966 | 5' | -59.6 | NC_002512.2 | + | 137966 | 0.66 | 0.817959 |
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Target: 5'- -aGu-CGGCcgccgacGGCGCGCCGCCGCc-- -3' miRNA: 3'- uaCuuGCCGa------CCGUGUGGCGGCGcaa -5' |
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| 8966 | 5' | -59.6 | NC_002512.2 | + | 207395 | 0.66 | 0.823775 |
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Target: 5'- gAUGGACGGCUGcuGCGCGCUcauguccauggccuGCUGCa-- -3' miRNA: 3'- -UACUUGCCGAC--CGUGUGG--------------CGGCGcaa -5' |
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| 8966 | 5' | -59.6 | NC_002512.2 | + | 220133 | 0.66 | 0.826243 |
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Target: 5'- -gGGGCGGCgGGUccgGCuCCGCCGCc-- -3' miRNA: 3'- uaCUUGCCGaCCG---UGuGGCGGCGcaa -5' |
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| 8966 | 5' | -59.6 | NC_002512.2 | + | 121142 | 0.66 | 0.826243 |
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Target: 5'- --cGACGGC-GGCcgagACGCCGgCCGCGg- -3' miRNA: 3'- uacUUGCCGaCCG----UGUGGC-GGCGCaa -5' |
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| 8966 | 5' | -59.6 | NC_002512.2 | + | 71011 | 0.66 | 0.783311 |
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Target: 5'- -aGGACGGggGGC-CGCCGCCGg--- -3' miRNA: 3'- uaCUUGCCgaCCGuGUGGCGGCgcaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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