miRNA: 3'- uaCUuG-CCGA--CCguGUGGCGGCGCaa -5'
miRNA: 3'- -UACUu--GCCG--------ACCGu-----GUGGCGGCGCAa -5'
miRNA: 3'- uaCUUGCCGaCCGUGu-GGCG-GCGCaa -5'
miRNA: 3'- uaCUUGCCGaCCGugUGGCGG-CGCaa -5'
miRNA: 3'- uaCU---UGCCGACCGUgugGCGGCGCaa -5'
Results 81 - 85 of 85 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8966 | 5' | -59.6 | NC_002512.2 | + | 224619 | 0.66 | 0.783311 |
|
Target: 5'- -cGAcCgGGCUcgGGguCGCCGCCGCGg- -3' miRNA: 3'- uaCUuG-CCGA--CCguGUGGCGGCGCaa -5' |
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| 8966 | 5' | -59.6 | NC_002512.2 | + | 226799 | 0.67 | 0.746623 |
|
Target: 5'- cGUGGucuCGGCcgccgccgUGGCcgccgcCGCCGCCGCGUc -3' miRNA: 3'- -UACUu--GCCG--------ACCGu-----GUGGCGGCGCAa -5' |
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| 8966 | 5' | -59.6 | NC_002512.2 | + | 227192 | 0.73 | 0.413802 |
|
Target: 5'- -gGAGCGGCaGGCGCucCCGCgCGCGg- -3' miRNA: 3'- uaCUUGCCGaCCGUGu-GGCG-GCGCaa -5' |
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| 8966 | 5' | -59.6 | NC_002512.2 | + | 227671 | 0.66 | 0.783311 |
|
Target: 5'- -aGAGCGGC-GGC--GCCGCCgGCGa- -3' miRNA: 3'- uaCUUGCCGaCCGugUGGCGG-CGCaa -5' |
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| 8966 | 5' | -59.6 | NC_002512.2 | + | 227672 | 0.67 | 0.746623 |
|
Target: 5'- -cGAagcGCGGCUGGCGa--CGCUGCGc- -3' miRNA: 3'- uaCU---UGCCGACCGUgugGCGGCGCaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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