Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
897 | 3' | -51.6 | NC_000899.1 | + | 4074 | 0.66 | 0.864957 |
Target: 5'- gAUCCGGaUCGAAaaggGCCaagGCCGAC-ACGAc -3' miRNA: 3'- -UAGGUCaAGCUU----UGG---CGGUUGcUGCU- -5' |
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897 | 3' | -51.6 | NC_000899.1 | + | 18097 | 0.66 | 0.856403 |
Target: 5'- --gCAGcgUC--AACCGUCGACGACGAa -3' miRNA: 3'- uagGUCa-AGcuUUGGCGGUUGCUGCU- -5' |
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897 | 3' | -51.6 | NC_000899.1 | + | 44268 | 0.66 | 0.838541 |
Target: 5'- -gUUGGUUCGAAAauGCCAGCGGUGAu -3' miRNA: 3'- uaGGUCAAGCUUUggCGGUUGCUGCU- -5' |
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897 | 3' | -51.6 | NC_000899.1 | + | 1454 | 0.66 | 0.838541 |
Target: 5'- uAUCUGccUCGAGagcgGCCGCCAguugaACGGCGAa -3' miRNA: 3'- -UAGGUcaAGCUU----UGGCGGU-----UGCUGCU- -5' |
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897 | 3' | -51.6 | NC_000899.1 | + | 38804 | 0.67 | 0.778623 |
Target: 5'- aGUCCGGcgCGGgugcaguGGCgGCCAACGGCc- -3' miRNA: 3'- -UAGGUCaaGCU-------UUGgCGGUUGCUGcu -5' |
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897 | 3' | -51.6 | NC_000899.1 | + | 13240 | 0.68 | 0.747823 |
Target: 5'- cUCCAaaacGUUCGGAACgcgagcgcgggCGCCGGCGGgGAa -3' miRNA: 3'- uAGGU----CAAGCUUUG-----------GCGGUUGCUgCU- -5' |
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897 | 3' | -51.6 | NC_000899.1 | + | 14253 | 0.68 | 0.725938 |
Target: 5'- cGUCCGGUgCGGuuCCGUCAGaagcgcauCGGCGAg -3' miRNA: 3'- -UAGGUCAaGCUuuGGCGGUU--------GCUGCU- -5' |
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897 | 3' | -51.6 | NC_000899.1 | + | 16178 | 0.68 | 0.725938 |
Target: 5'- aAUCCGGagccUGAcauGCCGCCGACG-CGAg -3' miRNA: 3'- -UAGGUCaa--GCUu--UGGCGGUUGCuGCU- -5' |
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897 | 3' | -51.6 | NC_000899.1 | + | 10365 | 0.69 | 0.692383 |
Target: 5'- -aCCGGUaggcgCGGAACCGCCgGGCaACGAa -3' miRNA: 3'- uaGGUCAa----GCUUUGGCGG-UUGcUGCU- -5' |
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897 | 3' | -51.6 | NC_000899.1 | + | 9787 | 0.7 | 0.635358 |
Target: 5'- aGUCUuGUUCGuAACCGCCGAUGGaGAc -3' miRNA: 3'- -UAGGuCAAGCuUUGGCGGUUGCUgCU- -5' |
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897 | 3' | -51.6 | NC_000899.1 | + | 9890 | 0.7 | 0.623897 |
Target: 5'- -gCCAGUgccccuaCGAGGCCGUCAACGAa-- -3' miRNA: 3'- uaGGUCAa------GCUUUGGCGGUUGCUgcu -5' |
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897 | 3' | -51.6 | NC_000899.1 | + | 13667 | 0.72 | 0.489751 |
Target: 5'- -aCCGGggUCGcgugcuGACCGCCGACGACu- -3' miRNA: 3'- uaGGUCa-AGCu-----UUGGCGGUUGCUGcu -5' |
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897 | 3' | -51.6 | NC_000899.1 | + | 5489 | 1.07 | 0.002543 |
Target: 5'- cAUCCAGUUCGAAACCGCCAACGACGAc -3' miRNA: 3'- -UAGGUCAAGCUUUGGCGGUUGCUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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