Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8981 | 3' | -52.2 | NC_002512.2 | + | 213022 | 0.68 | 0.969725 |
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Target: 5'- cCCUCUCGAGGUgCGuccGCCgGCUGCGgggGAu -3' miRNA: 3'- -GGGGAGUUCCA-GU---UGG-CGAUGUa--CUu -5' |
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| 8981 | 3' | -52.2 | NC_002512.2 | + | 132151 | 0.68 | 0.979999 |
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Target: 5'- gCCaCCUCGAGGUCGgugAgCGCggcguCGUGAGc -3' miRNA: 3'- -GG-GGAGUUCCAGU---UgGCGau---GUACUU- -5' |
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| 8981 | 3' | -52.2 | NC_002512.2 | + | 220002 | 0.67 | 0.985804 |
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Target: 5'- cCCCCUCGcccggcGGGUCGagagGCCGCUcCGUc-- -3' miRNA: 3'- -GGGGAGU------UCCAGU----UGGCGAuGUAcuu -5' |
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| 8981 | 3' | -52.2 | NC_002512.2 | + | 100809 | 0.67 | 0.990222 |
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Target: 5'- gUCCUCGGGGUCGACCuggacgaggauGUUcaucgGCGUGAc -3' miRNA: 3'- gGGGAGUUCCAGUUGG-----------CGA-----UGUACUu -5' |
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| 8981 | 3' | -52.2 | NC_002512.2 | + | 96587 | 0.66 | 0.991312 |
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Target: 5'- cCCgCCUCGGGGUCcucugggGACCGgaGCGcGGAg -3' miRNA: 3'- -GG-GGAGUUCCAG-------UUGGCgaUGUaCUU- -5' |
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| 8981 | 3' | -52.2 | NC_002512.2 | + | 152407 | 0.66 | 0.991427 |
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Target: 5'- gCCCaCAGGG-CGAUCGCUGCGUc-- -3' miRNA: 3'- gGGGaGUUCCaGUUGGCGAUGUAcuu -5' |
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| 8981 | 3' | -52.2 | NC_002512.2 | + | 40358 | 0.66 | 0.993482 |
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Target: 5'- uCCCCUCGGGGcCugcGCCGCU-CGg--- -3' miRNA: 3'- -GGGGAGUUCCaGu--UGGCGAuGUacuu -5' |
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| 8981 | 3' | -52.2 | NC_002512.2 | + | 110697 | 0.66 | 0.99435 |
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Target: 5'- gCCgCCgCAGGGcCAggGCCaGCUGCAUGGc -3' miRNA: 3'- -GG-GGaGUUCCaGU--UGG-CGAUGUACUu -5' |
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| 8981 | 3' | -52.2 | NC_002512.2 | + | 194577 | 1.09 | 0.009997 |
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Target: 5'- gCCCCUCAAGGUCAACCGCUACAUGAAg -3' miRNA: 3'- -GGGGAGUUCCAGUUGGCGAUGUACUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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