Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8984 | 3' | -56.9 | NC_002512.2 | + | 121335 | 0.66 | 0.936441 |
Target: 5'- -gGUGaccUCGCUCCGgCggCGGGGcCGUUGg -3' miRNA: 3'- agUACa--AGUGAGGCgG--GCCCC-GCAAC- -5' |
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8984 | 3' | -56.9 | NC_002512.2 | + | 124014 | 0.66 | 0.931622 |
Target: 5'- gCGUGUUCGCgCUGCCCcuGGGCc--- -3' miRNA: 3'- aGUACAAGUGaGGCGGGc-CCCGcaac -5' |
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8984 | 3' | -56.9 | NC_002512.2 | + | 123677 | 0.66 | 0.931622 |
Target: 5'- cUCggGgaagCGgUCCGUCgcggCGGGGCGUUGg -3' miRNA: 3'- -AGuaCaa--GUgAGGCGG----GCCCCGCAAC- -5' |
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8984 | 3' | -56.9 | NC_002512.2 | + | 49454 | 0.66 | 0.931622 |
Target: 5'- -------gGCgCCGCCCGGGGCGc-- -3' miRNA: 3'- aguacaagUGaGGCGGGCCCCGCaac -5' |
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8984 | 3' | -56.9 | NC_002512.2 | + | 215403 | 0.66 | 0.92658 |
Target: 5'- cCAUGUUCGCcCUGCCCuggcccaaGGGCGc-- -3' miRNA: 3'- aGUACAAGUGaGGCGGGc-------CCCGCaac -5' |
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8984 | 3' | -56.9 | NC_002512.2 | + | 147924 | 0.66 | 0.921315 |
Target: 5'- ------cCAC-CCGCCCGGGGCu--- -3' miRNA: 3'- aguacaaGUGaGGCGGGCCCCGcaac -5' |
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8984 | 3' | -56.9 | NC_002512.2 | + | 137192 | 0.67 | 0.915826 |
Target: 5'- gCGUcgUCGgUCCGCCCGguGGGCGg-- -3' miRNA: 3'- aGUAcaAGUgAGGCGGGC--CCCGCaac -5' |
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8984 | 3' | -56.9 | NC_002512.2 | + | 117964 | 0.67 | 0.906584 |
Target: 5'- gCGUGgaCGCUCCccgccuggccgugcuGCUCGGGGUGcUGg -3' miRNA: 3'- aGUACaaGUGAGG---------------CGGGCCCCGCaAC- -5' |
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8984 | 3' | -56.9 | NC_002512.2 | + | 108926 | 0.67 | 0.898037 |
Target: 5'- gUCcUGUUCAUgaUCUGCCUGGcGGUGUc- -3' miRNA: 3'- -AGuACAAGUG--AGGCGGGCC-CCGCAac -5' |
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8984 | 3' | -56.9 | NC_002512.2 | + | 123428 | 0.67 | 0.891672 |
Target: 5'- uUCGUGUUCGCgaUCC-CCCGacaGGCGUUc -3' miRNA: 3'- -AGUACAAGUG--AGGcGGGCc--CCGCAAc -5' |
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8984 | 3' | -56.9 | NC_002512.2 | + | 21222 | 0.68 | 0.878308 |
Target: 5'- cCcgGg--ACgCCGCCCGGGGCGg-- -3' miRNA: 3'- aGuaCaagUGaGGCGGGCCCCGCaac -5' |
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8984 | 3' | -56.9 | NC_002512.2 | + | 5490 | 0.68 | 0.849163 |
Target: 5'- cUCGUGgcgCGCgUCCGCgCCGGGGUc--- -3' miRNA: 3'- -AGUACaa-GUG-AGGCG-GGCCCCGcaac -5' |
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8984 | 3' | -56.9 | NC_002512.2 | + | 126477 | 0.69 | 0.833466 |
Target: 5'- gUCGUcgUCGCgUCGCCCGGauccccGGCGUUGg -3' miRNA: 3'- -AGUAcaAGUGaGGCGGGCC------CCGCAAC- -5' |
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8984 | 3' | -56.9 | NC_002512.2 | + | 73842 | 0.69 | 0.828621 |
Target: 5'- cCGUGUUCACgugaucaaagaCCGCCCGGGaucacacaacgaccuGgGUUGg -3' miRNA: 3'- aGUACAAGUGa----------GGCGGGCCC---------------CgCAAC- -5' |
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8984 | 3' | -56.9 | NC_002512.2 | + | 122032 | 0.7 | 0.782503 |
Target: 5'- aUCGgcgcUCGCgcaCGCCCGGGGCGg-- -3' miRNA: 3'- -AGUaca-AGUGag-GCGGGCCCCGCaac -5' |
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8984 | 3' | -56.9 | NC_002512.2 | + | 107155 | 0.7 | 0.755217 |
Target: 5'- aUCAg---CGCguuUCCGCCCGggaGGGCGUUGa -3' miRNA: 3'- -AGUacaaGUG---AGGCGGGC---CCCGCAAC- -5' |
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8984 | 3' | -56.9 | NC_002512.2 | + | 77337 | 0.71 | 0.727019 |
Target: 5'- -------gGCUCCuGaCCCGGGGCGUUGa -3' miRNA: 3'- aguacaagUGAGG-C-GGGCCCCGCAAC- -5' |
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8984 | 3' | -56.9 | NC_002512.2 | + | 206304 | 0.71 | 0.698133 |
Target: 5'- cUCGUGUUCGCUCuCGCCCaGcGGCc--- -3' miRNA: 3'- -AGUACAAGUGAG-GCGGGcC-CCGcaac -5' |
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8984 | 3' | -56.9 | NC_002512.2 | + | 99533 | 0.73 | 0.589829 |
Target: 5'- -gGUGagCGgUCCGCCCGGGGuCGUg- -3' miRNA: 3'- agUACaaGUgAGGCGGGCCCC-GCAac -5' |
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8984 | 3' | -56.9 | NC_002512.2 | + | 191440 | 1.09 | 0.003797 |
Target: 5'- gUCAUGUUCACUCCGCCCGGGGCGUUGa -3' miRNA: 3'- -AGUACAAGUGAGGCGGGCCCCGCAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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