Results 1 - 20 of 54 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8986 | 5' | -56.8 | NC_002512.2 | + | 180311 | 0.66 | 0.946856 |
Target: 5'- aGGCGcagcGGcGUCG-UCAGGUCCC-GCUg -3' miRNA: 3'- -CCGCu---CCuCGGCaAGUUCAGGGaCGA- -5' |
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8986 | 5' | -56.8 | NC_002512.2 | + | 80650 | 0.66 | 0.946856 |
Target: 5'- cGGCGGcGGcGCCGggUCcgucGGUCCCgcgGCg -3' miRNA: 3'- -CCGCU-CCuCGGCa-AGu---UCAGGGa--CGa -5' |
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8986 | 5' | -56.8 | NC_002512.2 | + | 223919 | 0.66 | 0.946856 |
Target: 5'- gGGCGAGaggucGAGCCGgaCGGGgaggucgaUCCCUGg- -3' miRNA: 3'- -CCGCUC-----CUCGGCaaGUUC--------AGGGACga -5' |
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8986 | 5' | -56.8 | NC_002512.2 | + | 107219 | 0.66 | 0.946856 |
Target: 5'- cGGcCGGGGAGCCGcggUCAccgucGUCCg-GCUc -3' miRNA: 3'- -CC-GCUCCUCGGCa--AGUu----CAGGgaCGA- -5' |
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8986 | 5' | -56.8 | NC_002512.2 | + | 159888 | 0.66 | 0.942542 |
Target: 5'- gGGgGuGGAGgUGUgUCGAcggcucuuGUCCCUGCUg -3' miRNA: 3'- -CCgCuCCUCgGCA-AGUU--------CAGGGACGA- -5' |
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8986 | 5' | -56.8 | NC_002512.2 | + | 48701 | 0.66 | 0.942542 |
Target: 5'- gGGCGAGGAccaCgCGUUCGAcUCcuaCCUGCUg -3' miRNA: 3'- -CCGCUCCUc--G-GCAAGUUcAG---GGACGA- -5' |
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8986 | 5' | -56.8 | NC_002512.2 | + | 127292 | 0.66 | 0.942542 |
Target: 5'- cGGCGAGGAGaggaggaaacgCGUgcgCGAGaCCCUGg- -3' miRNA: 3'- -CCGCUCCUCg----------GCAa--GUUCaGGGACga -5' |
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8986 | 5' | -56.8 | NC_002512.2 | + | 124961 | 0.66 | 0.942098 |
Target: 5'- cGGaCGAGGAgGCCGUcgCGGacuucgccaaccuGUCCCUGg- -3' miRNA: 3'- -CC-GCUCCU-CGGCAa-GUU-------------CAGGGACga -5' |
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8986 | 5' | -56.8 | NC_002512.2 | + | 196177 | 0.66 | 0.942098 |
Target: 5'- gGGCGAgcccgagGGGGCgaCGgcCGGGUCCCUgGCg -3' miRNA: 3'- -CCGCU-------CCUCG--GCaaGUUCAGGGA-CGa -5' |
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8986 | 5' | -56.8 | NC_002512.2 | + | 109047 | 0.66 | 0.938006 |
Target: 5'- aGGgGAGGAaguacgcgucGCCcagCAGGUCCCcGCg -3' miRNA: 3'- -CCgCUCCU----------CGGcaaGUUCAGGGaCGa -5' |
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8986 | 5' | -56.8 | NC_002512.2 | + | 189911 | 0.66 | 0.933248 |
Target: 5'- cGGCGAGGAcgGCgGcUCcGGUCUCgGCg -3' miRNA: 3'- -CCGCUCCU--CGgCaAGuUCAGGGaCGa -5' |
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8986 | 5' | -56.8 | NC_002512.2 | + | 164258 | 0.66 | 0.933248 |
Target: 5'- uGGCG-GGGGCCG-UCGGGUCgaucgCCUcggGCUu -3' miRNA: 3'- -CCGCuCCUCGGCaAGUUCAG-----GGA---CGA- -5' |
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8986 | 5' | -56.8 | NC_002512.2 | + | 116892 | 0.66 | 0.931777 |
Target: 5'- uGGCGGcGGGGCCGgggCGGgcuccucggacggcGUCCCcgGCUu -3' miRNA: 3'- -CCGCU-CCUCGGCaa-GUU--------------CAGGGa-CGA- -5' |
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8986 | 5' | -56.8 | NC_002512.2 | + | 159359 | 0.66 | 0.92359 |
Target: 5'- cGGCGucGGGGUCGgggcgucccauucgCGGGUCCCgcgGCg -3' miRNA: 3'- -CCGCu-CCUCGGCaa------------GUUCAGGGa--CGa -5' |
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8986 | 5' | -56.8 | NC_002512.2 | + | 135807 | 0.66 | 0.923059 |
Target: 5'- cGGCGGGGAcGCCGac--GGUCCCg--- -3' miRNA: 3'- -CCGCUCCU-CGGCaaguUCAGGGacga -5' |
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8986 | 5' | -56.8 | NC_002512.2 | + | 81946 | 0.66 | 0.923059 |
Target: 5'- gGGCgGAGGGGCCGgcgcgcggUGAGcCCCggGCg -3' miRNA: 3'- -CCG-CUCCUCGGCaa------GUUCaGGGa-CGa -5' |
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8986 | 5' | -56.8 | NC_002512.2 | + | 96881 | 0.67 | 0.917628 |
Target: 5'- cGGCGcacuucccGGGGGCCGggUuc--CCCUGCUa -3' miRNA: 3'- -CCGC--------UCCUCGGCaaGuucaGGGACGA- -5' |
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8986 | 5' | -56.8 | NC_002512.2 | + | 133878 | 0.67 | 0.917628 |
Target: 5'- cGGCucGAGGAGCgGcUCGgccggcGGUCCCggGCg -3' miRNA: 3'- -CCG--CUCCUCGgCaAGU------UCAGGGa-CGa -5' |
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8986 | 5' | -56.8 | NC_002512.2 | + | 221616 | 0.67 | 0.917072 |
Target: 5'- cGGCGAGGAGggagcggguccacCCGgc--GG-CCCUGCUc -3' miRNA: 3'- -CCGCUCCUC-------------GGCaaguUCaGGGACGA- -5' |
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8986 | 5' | -56.8 | NC_002512.2 | + | 113278 | 0.67 | 0.906096 |
Target: 5'- cGGCGGGGcAGCCGcggCGGGccUCCC-GCc -3' miRNA: 3'- -CCGCUCC-UCGGCaa-GUUC--AGGGaCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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