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miRNA: 3'- cACGuGCUGCAGCCGa-----GGCUCGuGUG- -5'
miRNA: 3'- cACGUGCUGCAGCCGaGGCu-CGUGUG- -5'
miRNA: 3'- -CACGUGCUGCAGCCGAGGCUCGUGUG- -5'
Results 121 - 123 of 123 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 8987 | 5' | -57.8 | NC_002512.2 | + | 72926 | 0.77 | 0.355212 |
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Target: 5'- aUGC-CGACGUCGGCgaacggCCGGGC-CACc -3' miRNA: 3'- cACGuGCUGCAGCCGa-----GGCUCGuGUG- -5' |
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| 8987 | 5' | -57.8 | NC_002512.2 | + | 114780 | 0.79 | 0.266293 |
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Target: 5'- cUGCGCGAUGUUGGC-CCGcuGCACGCg -3' miRNA: 3'- cACGUGCUGCAGCCGaGGCu-CGUGUG- -5' |
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| 8987 | 5' | -57.8 | NC_002512.2 | + | 190274 | 1.08 | 0.003601 |
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Target: 5'- gGUGCACGACGUCGGCUCCGAGCACACc -3' miRNA: 3'- -CACGUGCUGCAGCCGAGGCUCGUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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