Results 121 - 123 of 123 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8987 | 5' | -57.8 | NC_002512.2 | + | 170377 | 0.66 | 0.918636 |
Target: 5'- cGUGCGCGGCGcCGcGCugcacuUCUG-GCACAg -3' miRNA: 3'- -CACGUGCUGCaGC-CG------AGGCuCGUGUg -5' |
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8987 | 5' | -57.8 | NC_002512.2 | + | 47449 | 0.66 | 0.918636 |
Target: 5'- -cGCACGGCGgaGGCcgaggCCGAGUucCGCa -3' miRNA: 3'- caCGUGCUGCagCCGa----GGCUCGu-GUG- -5' |
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8987 | 5' | -57.8 | NC_002512.2 | + | 181384 | 0.66 | 0.885883 |
Target: 5'- -cGCGCGuCGUCGuGCgcccuggccuccgCCGGGgACGCg -3' miRNA: 3'- caCGUGCuGCAGC-CGa------------GGCUCgUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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